FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120223
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30900.043.743361
GGTATCA11650.039.956961
TCAACGC36600.036.8103524
TATCAAC37250.036.538032
ATCAACG37400.036.2072263
CAACGCA38300.035.446375
CTTACCG400.002612326334.4567533
AACGCAG40100.033.7693336
CAGAGTA42900.031.72732510
ACGCAGA42900.031.645597
AGAGTAC43400.031.44120211
CGCAGAG43650.031.2597338
CTAACGA500.005407796229.69825668
GCAGAGT47200.028.9086279
TCTAACG500.006485617328.61396267
AGTACGG40150.027.72111313
GAGTACG40350.027.5837112
GTGGTAT4400.027.4283641
TACGGGG19450.025.86586215
CCGCTAT1751.1109189E-525.23599270
Produced by FastQC (version 0.11.5)