FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216142
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATG303.481337E-582.58485470
GACCGGA551.0276248E-556.30785870
GGTATCA2400.044.5310971
GTATCAA5900.043.2406921
TATCAAC7050.035.673452
CCTACGT501.7527933E-434.83867651
ACGATTA501.811471E-434.64413540
ATCAACG7300.034.451763
TCAACGC7400.033.986194
CAACGCA7600.033.091825
AACGCAG7700.032.662066
TACGTGA553.0547552E-431.6937753
TCCTACG553.0715833E-431.66411650
AGAGTAC7950.031.63494911
CGCAGAG8000.031.4372278
CAGACCG603.489046E-430.98220868
ACGCAGA8450.029.7630587
GCAGAGT8450.029.7630589
CAGAGTA8450.029.76305810
CGATTAA605.263027E-428.88355441
Produced by FastQC (version 0.11.5)