FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432477
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT44840.31302422307653105No Hit
GTATAACAGTGCCCCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT41840.2920814784460763No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT39120.2730933899811306No Hit
GTCTACTATTGTGCGAGGCATCAGCCTTGGGTGGTAGCTTACACCATAAC35870.25040541663147126No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT35510.24789228727581664No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACGATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAA34930.24384335664726203No Hit
ATAGTAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAAGTTCAGGGAGA34150.2383982430433438No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC30400.2122198122552753No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC28660.20007302036961153No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27490.19190534996373415No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25830.18031703126821583No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24060.16796081193624748No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22720.15860638600131102No Hit
CTGTTATACTGTTGACAGGAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC22270.15546497430674278No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21960.15330089069492914No Hit
CAGTATAACAGTGCCCCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT21900.15288203580232004No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA21700.1514858528269564No Hit
AGCTTACACCATAACGGGGTACTTCGATCTCTGGGGCCGTGGCACCCTGG21150.14764634964470633No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20830.1454124568841245No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG20420.142550281784629No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC20150.14066543476788806No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG20130.1405258164703517No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20090.14024657987527897No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACGATATCCGTAGACACGTCC20070.1401069615777426No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA19690.13745421392455168No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19530.13633726754426076No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC19520.13626745839549256No Hit
GTGTAAGCTACCACCCAAGGCTGATGCCTCGCACAATAGTAGACAGCCGT19310.13480146627136072No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18880.13179967287432887No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18780.13110158138664704No Hit
CACCTGCACTGTCTCTGTTGACTCCATCAGCAGTAGTAGTTACTACTGGG18580.1297053984112834No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG18250.12740169650193336No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17670.12335276587337876No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17660.12328295672461059No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT17560.12258486523692876No Hit
GCCTTGGGTGGTAGCTTACACCATAACGGGGTACTTCGATCTCTGGGGCC17330.12097925481526056No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17300.120769827368956No Hit
GTCTCTGTTGACTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG17190.120001926732506No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG16690.11651146929409688No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16070.11218330207046953No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG15520.10834379888821949No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15520.10834379888821949No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT15510.10827398973945132No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC15420.10764570740053768No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA15240.1063891427227104No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA15180.10597028783010129No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA14960.10443448655720128No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC14840.10359677677198306No Hit
GTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGA14790.10324773102814216No Hit
GTAGTAGGTGGTCCCACGATAATAGATAATACCAATCCATTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC14750.10296849443306942No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14660.10234021209415578No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA14650.10227040294538761No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG14600.10192135720154669No Hit
CTACTATTGTGCGAGGCATCAGCCTTGGGTGGTAGCTTACACCATAACGGGGTACTTCGATCTCTGGGGCCGTGG14550.10157231145770577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATA150.002232488269.9407958
GTATCAA19650.037.6760061
GTGGTAT3350.037.003831
TACGGAT12650.036.1742770
TATCGAT553.2285563E-431.41127640
TATCAAC23750.030.7133772
ATCAACG24050.030.330263
TCAACGC24300.029.8766254
CAACGCA24550.029.572385
AACGCAG25200.028.80966
GTATAGC605.4838473E-428.6950991
GTAAATC500.006861397528.28639465
GTATAAC15350.027.1435071
GGTATCA15600.026.7085131
TATAACA15550.026.5525472
CGCAGAG27500.026.5261178
AGAGTAC27650.026.38221411
CAGAGTA27600.026.30533810
GCAGAGT28750.025.4924899
TAACAGT16350.025.4637834
Produced by FastQC (version 0.11.5)