FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432477
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT45580.31819010008537657No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT42920.29962086651304004No Hit
GTATAACAGTGCCCCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT39550.2760951833781624No Hit
GTCTACTATTGTGCGAGGCATCAGCCTTGGGTGGTAGCTTACACCATAAC34530.24105099069653474No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACGATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAA33570.23434931241478918No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32160.22450622243847546No Hit
ATAGTAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAAGTTCAGGGAGA30440.21249904885034804No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC28460.19867683739424788No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27380.19113744932728413No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25410.17738504701995214No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25320.17675676468103851No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22830.15937428663776101No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22780.15902524089392012No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT22570.15755924876978827No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC20610.14387665561122448No Hit
AGCTTACACCATAACGGGGTACTTCGATCTCTGGGGCCGTGGCACCCTGG20330.14192199944571535No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20070.1401069615777426No Hit
CTGTTATACTGTTGACAGGAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC19990.13954848838759715No Hit
CAGTATAACAGTGCCCCCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT19970.13940887009006078No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19960.1393390609412926No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19680.1373844047757835No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA19660.13724478647824712No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19620.1369655498831744No Hit
GTGTAAGCTACCACCCAAGGCTGATGCCTCGCACAATAGTAGACAGCCGT19410.13549955775904254No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA19400.13542974861027438No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG19110.13340528329599707No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18880.13179967287432887No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC18880.13179967287432887No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18790.13117139053541524No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACGATATCCGTAGACACGTCC18610.12991482585758796No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18600.12984501670881976No Hit
CACCTGCACTGTCTCTGTTGACTCCATCAGCAGTAGTAGTTACTACTGGG17780.12412066650982878No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17720.12370181161721967No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17470.12195658289801511No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17430.12167734630294239No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG17360.12118868226156511No Hit
GTCTCTGTTGACTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG16420.11462662227735593No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT16380.1143473856822832No Hit
GCCTTGGGTGGTAGCTTACACCATAACGGGGTACTTCGATCTCTGGGGCC16360.11420776738474683No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA16140.11267196611184681No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16140.11267196611184681No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15950.11134559228525136No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15820.11043807335126497No Hit
GTAGTAGGTGGTCCCACGATAATAGATAATACCAATCCATTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC15590.10883246292959677No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG15430.10771551654930585No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA15140.10569105123502856No Hit
GTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGA15070.10520238719365128No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14550.10157231145770577No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC14520.10136288401140124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19150.041.4830361
GTGGTAT2850.039.8195571
TACGGAT9550.036.12241770
TATCAAC22500.035.1440162
ATCAACG22800.034.2292253
CAACGCA23650.032.9989975
TCAACGC23550.032.9931344
AACGCAG24250.032.325436
CAGAGTA25950.030.0931410
AGAGTAC26200.029.80598811
CGCAGAG26400.029.577098
GTCCTAT703.93681E-529.4768141
TGTTGCG604.8682856E-429.28479262
TTAACGG605.433571E-428.74029735
TGTTAGA8100.028.6655270
GCAGAGT28300.027.5932779
GGTATCA13300.027.1496981
TACAATA1000.009367526526.53608370
TGATACT658.8360684E-426.4461654
TTCGGGG20100.026.0740170
Produced by FastQC (version 0.11.5)