FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688143
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTATACTGCATCCAATTTGCAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA40500.23990858594325243No Hit
GTATAGGTCAGGAGCTTAGGGGCACTCCCTGGTTTCTGCTGAAACCAATT35010.2073876442931671No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25640.15188286774283932No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19760.11705169526515229No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19470.11533383131642283No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18270.1082254287699561No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18150.10751458851530943No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18090.10715916838798609No Hit
GACTTACAGTACCCCTCGGACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAACTGTGGCTGCACCAT17570.10407886061785049No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16910.1001692392172938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.039.9151461
GTATCAA24450.038.5683141
TATCAAC28300.032.934722
ATCAACG29250.031.8650463
TCAACGC29700.031.3822424
CAACGCA30100.030.9652045
AACGCAG31250.030.0466146
CGACAAT703.8187143E-529.60914432
TGGTATC4450.029.478152
GTGGTAT4750.029.0879611
AGAGTAC33650.027.69844411
TGACTTG14200.027.10788270
TCGTATG906.7403653E-626.91862340
CGCAGAG35700.026.3972238
ACGCAGA35700.026.300537
GTGTATA1759.094947E-1225.64377613
GCAGAGT37650.025.121729
CAGAGTA37750.025.05591610
GTAGTTT3151.6007107E-1024.4401270
CGTATGC1151.6466292E-624.0855841
Produced by FastQC (version 0.11.5)