FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704335
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21300.043.733981
GTATCAA51000.040.5073241
TCAACGC61000.033.5168724
ATCAACG61250.033.380073
TATCAAC62550.032.7412532
CAACGCA62850.032.5302965
AACGCAG64050.032.137316
ACGCAGA66800.030.7745867
GTGGTAT7500.030.7073821
CGCAGAG69000.029.795998
AGAGTAC69700.029.44828811
CAGAGTA70250.029.21773110
GCAGAGT76050.027.079849
AGTACGG56750.024.47563613
TGGTATC8900.024.3235452
GAGTACG57700.024.31112
AGTACTT27300.023.42441713
GAGTACT28050.023.41094812
TACGGGG26600.022.48718615
GTACTTT28950.022.3262114
Produced by FastQC (version 0.11.5)