FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771453
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCCCCAACAATAAAAACTACTTAGCTTGGTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACT62500.8101595301333977No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42720.55376024203678No Hit
GGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAA38010.49270661984592706No Hit
GTATAAAAGAGTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGTCACAGCCAGGGAGTCTGGAGA36410.4719665358745121No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35720.4630223746618394No Hit
GTCCTGGCCTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGGGCTG32310.4188200706977612No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27250.35322955513816134No Hit
ATATTATAGTTTTCCCTGGGGGTTCGGCCAGGGGACCCAGGTGGAGATCAAACGAACTGTGGCTGCACCAT26860.34817415967012894No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24910.32289718232996695No Hit
GTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCTGA24210.3138233955924729No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24020.31136051062086734No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23790.3083791235499765No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23570.3055273620039069No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA22480.29139817979838045No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG21790.28245401858570773No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20910.2710469724014295No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20310.2632694409121489No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19910.25808441991929515No Hit
CTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC19840.25717704124554575No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19210.24901063318180108No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18490.23967759539466432No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18360.2379924635719869No Hit
CTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGGGCTGTATAAAAGAG18320.23747396147270153No Hit
GTCTCCAGACTCCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGACTCTTTTATA18150.23527032755073868No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18110.2347518254514533No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17850.23138156180609837No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17800.23073343418199163No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTGGGTCCCCTGGCCGAACCCC17550.22749279606145806No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17270.22386328136646044No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17250.22360403031681775No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16380.21232660965736083No Hit
GTTTATTACTGTCAACAATATTATAGTTTTCCCTGGGGGTTCGGCCAGGGGACCCAGGTGGAGATCAAACGAA15940.20662308656522174No Hit
GTCAGGGACCCCGGATTCTCGGGTAGACGCCCAGTAAAAGAGTAGTTTTGGAGGCTGTCCTGGCCTCTGCTGGTA15380.19936405717522648No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15070.19534566590576485No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14620.1895125172888044No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14350.1860126281186281No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13850.17953135187756092No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13690.17745734348041942No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12900.16721692701953328No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12830.16630954834578388No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12800.16592067177131983No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12600.16332816127489297No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12440.16125415287775147No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12070.15645800845936175No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTTTT11880.1539951234877562No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC11650.1510137364168653No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11470.14868047697008113No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11390.14764347277151038No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTG11340.14699534514740367No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11320.14673609409776098No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11050.1432362049275847No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10990.14245845177865665No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10810.14012519233187246No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGACTCTGGGCGAGAGGG10740.13921781365812305No Hit
GTAAAAGAGTAGTTTTGGAGGCTGTCCTGGCCTCTGCTGGTACCAAGCTA10730.1390881881333017No Hit
GTTTTGGAGGCTGTCCTGGCCTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGGGCTGTATAAAAGAGT10720.13895856260848036No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTTTTCCCT10710.138828937083659No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10680.138440060509195No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10580.13714380526098155No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTTTTCCCTGGGGGTT10410.1349401713390187No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA10340.1340327926652693No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10280.13325503951634124No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10280.13325503951634124No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10040.13014402692062899No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10030.13001440139580764No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT9970.12923664824687958No Hit
CTCCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGACTCTTTTATACAGCCCCAA9900.12832926957313018No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT9900.12832926957313018No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTGGGTCC9880.1280700185234875No Hit
CCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT9760.12651451222563137No Hit
CCCAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT9600.12444050382848988No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9510.12327387410509778No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG9480.12288499753063375No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT9400.12184799333206299No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9370.12145911675759896No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9350.12119986570795628No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC9280.12029248703420688No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9220.11951473388527882No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9210.11938510836045747No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9170.1188666062611721No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8990.11653334681438791No Hit
CTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCT8860.11484821499171045No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC8840.11458896394206776No Hit
GGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGA8810.11420008736760373No Hit
CTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA8770.11368158526831836No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8520.11044094714778477No Hit
GCAGAGGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCTGACCGATT8510.11031132162296342No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8410.10901506637474999No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8410.10901506637474999No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8320.10784843665135788No Hit
GTATCAACGCAGAGTACGGGGGAGCTACAACAGGCAGGCAGGGGCAGCAA8300.1075891856017152No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCTCGGGTAGACGCCCAGTAAAAGAGTAGTTTTGGAGGCTGTCCTGGC8220.10655218140314446No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA8190.10616330482868042No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG8120.10525592615493103No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7960.10318191775778954No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7790.10097828383582667No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7770.10071903278618399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATT150.002323060369.2398443
CGAATAT309.221578E-657.3959634
GGTATCA7950.047.0968671
TAAGGCG2100.045.8720329
GACTAAG2100.045.83034526
ATCTCCG2200.043.68760710
CTTATAC2300.043.311461
AATCTCG1300.042.40125736
GTATCAA21450.042.27751
GTGGTAT2200.042.157391
AGACTAA2300.041.8342425
TCTCCGA2300.041.78814711
TCGTATG1000.041.44636540
TACACAT2350.040.9016885
TCCGAGC2350.040.89903613
GCCCACG2300.040.30616418
TGGTATC2300.040.2983252
GGCGAAT2500.039.92682332
TATACAC2500.039.8207173
TCGACCC350.001356984139.37259336
Produced by FastQC (version 0.11.5)