FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771453
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCCCCAACAATAAAAACTACTTAGCTTGGTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACT50420.6535718961492145No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40000.5185020992853745No Hit
GTATAAAAGAGTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGTCACAGCCAGGGAGTCTGGAGA34990.4535597113498813No Hit
GGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAA32490.4211533301445454No Hit
GTCCTGGCCTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGGGCTG30930.40093174827241584No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29250.3791546601024301No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29110.3773399027549313No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA27240.35309992961334No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26320.3411743813297764No Hit
ATATTATAGTTTTCCCTGGGGGTTCGGCCAGGGGACCCAGGTGGAGATCAAACGAACTGTGGCTGCACCAT25950.3363782369113867No Hit
CTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC22370.2899722990253457No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21940.2843984014580279No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21880.28362064830909983No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21770.28219476753606504No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21330.2764912444439259No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20390.26430644511071966No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG20270.26275093881286354No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19810.2567881646710817No Hit
GTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCTGA19800.25665853914626036No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19580.25380677760019077No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTGGGTCCCCTGGCCGAACCCC19440.25199202025269196No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18280.23695545937341614No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18100.23462219992663194No Hit
CTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGGGCTGTATAAAAGAG17990.23319631915359718No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17580.2278816726359221No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCTCGGGT16920.21932638799771342No Hit
GTTTATTACTGTCAACAATATTATAGTTTTCCCTGGGGGTTCGGCCAGGGGACCCAGGTGGAGATCAAACGAA16880.21880788589842803No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16490.21375249043039563No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC16360.21206735860771816No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16220.21025260126021936No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16120.2089563460120059No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16080.20843784391272055No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15960.20688233761486444No Hit
GTCAGGGACCCCGGATTCTCGGGTAGACGCCCAGTAAAAGAGTAGTTTTGGAGGCTGTCCTGGCCTCTGCTGGTA14870.19275315540933796No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC14630.18964214281362574No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14610.189382891763983No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14490.1878273854661269No Hit
GTCTCCAGACTCCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGACTCTTTTATA14410.18679038126755615No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13780.17862397320381151No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13770.17849434767899017No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13590.176161088232206No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA13440.17421670535988582No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG12560.16280965917560758No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTTTTCCCT12460.16151340392739416No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTTTT12330.15982827210471667No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA12190.15801351475721787No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11900.1542543745373989No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11850.1536062469132922No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11850.1536062469132922No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11760.1524396171899001No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11380.14751384724668903No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11320.14673609409776098No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11290.14634721752329696No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10750.1393474391829444No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10730.1390881881333017No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTTTTCCCTGGGGGTT10620.13766230736026694No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10580.13714380526098155No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10340.1340327926652693No Hit
GTTTTGGAGGCTGTCCTGGCCTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGGGCTGTATAAAAGAGT10240.13273653741705588No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9900.12832926957313018No Hit
GTAAAAGAGTAGTTTTGGAGGCTGTCCTGGCCTCTGCTGGTACCAAGCTA9880.1280700185234875No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCTCGGGTAGA9710.12586638460152466No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9700.1257367590767033No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA9430.12223686990652702No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG9410.12197761885688434No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9150.11860735521152942No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9110.11808885311224404No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT9040.11718147443849462No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9040.11718147443849462No Hit
CCCAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT9010.1167925978640306No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8910.11549634261581716No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8850.1147185894668891No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8680.11251495554492626No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTG8660.11225570449528356No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8570.11108907477189148No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC8550.1108298237222488No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT8420.10914469189957134No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGACTCTGGGCGAGAGGG8330.10797806217617925No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8320.10784843665135788No Hit
CTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCT8260.10707068350242982No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT8250.10694105797760849No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8240.10681143245278714No Hit
GTATCAACGCAGAGTACGGGGGAGCTACAACAGGCAGGCAGGGGCAGCAA8050.10434854748118162No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTGGGTCC7880.10214491355921877No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7880.10214491355921877No Hit
CCACCTGGGTCCCCTGGCCGAACCCCCAGGGAAAACTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAG7870.10201528803439742No Hit
GGTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAA7810.10123753488546937No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC7810.10123753488546937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA200.00748956951.51873828
GTGGTAT2600.046.1418531
TGGTATC2600.046.132882
GGTATCA8700.043.7324751
GTATCAA22200.041.0704081
TATCAAC25450.035.8186952
ATCAACG26250.034.7270743
TCAACGC26450.034.464494
ATTAATC400.002682304234.270143
CAACGCA27050.033.5733385
AACGCAG27150.033.327776
CGCAGAG28350.031.9212118
GACAGGT1400.031.8284631
AGAGTAC28700.031.41656111
TGTGCCG553.3784859E-431.16679410
ACGCAGA29350.030.9503977
CAGAGTA29550.030.62889110
GCAGAGT29600.030.5771529
CGGTCGT450.004766301330.474210
CCTTATA450.004775378430.4623472
Produced by FastQC (version 0.11.5)