FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415188
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT150.00235181669.019735
GTATCAA11600.043.918381
GGTATCA4600.041.9056661
CGAGACG756.4410175E-541.29805870
TCAACGC13600.037.9431464
ATCAACG13650.037.8041573
CAACGCA13800.037.3932385
AACGCAG14000.036.859056
TATCAAC14200.036.097652
AGAGTAC15150.034.06527311
ACGCAGA15550.033.4102557
CAGAGTA15850.032.5608110
CGCAGAG16200.032.2821048
GGCGTGT450.004438294630.9182248
GCGCACT450.004681437730.58304421
GCAGAGT17150.030.2932629
AGTACGG13950.029.59649713
GAGTACG14100.029.52565412
TACGGGG6700.029.26718715
CAACCGA1103.445166E-828.39657644
Produced by FastQC (version 0.11.5)