FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415188
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5410.13030241721822403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAA306.37624E-445.89783529
GGTATCA5650.040.1532331
GTATCAA12950.039.018681
TCAACGC14750.033.774724
ATCAACG14800.033.6606143
CAACGCA14950.033.3228845
TATCAAC15300.032.785152
AACGCAG15450.032.4707576
ACGCAGA16250.030.6755227
GTGGTAT2300.029.8900871
TACGGGA5250.029.47018615
CGCAGAG17050.029.2397278
AGAGTAC17300.029.01592411
CAGAGTA17200.028.98472810
ACGGGTA854.5429697E-628.31108316
AGTACGG15300.028.08977513
GAGTACG15350.027.99827612
CGAACCT1502.910383E-1127.94675453
GCAGAGT17950.027.7736689
GGCGATA500.007708344627.61534535
Produced by FastQC (version 0.11.5)