FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564969
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6210.1099175352983969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15200.040.4799921
GGTATCA6750.039.2119031
TATCAAC17700.034.3649442
ATCAACG18250.033.1409873
GTGGTAT2300.032.8795471
TCAACGC18300.032.862654
CAACGCA19100.031.4862045
AACGCAG20200.029.6067286
ACGGGTA703.9328872E-529.47653816
AGAGTAC20450.029.4389211
ACGCAGA20800.028.7679657
TAGGTAC605.544555E-428.6374533
CGCAGAG21100.028.5295078
TACGGGT2700.028.03083615
CAGAGTA21550.027.776610
AATAGCG1455.675247E-1026.65259462
GCAGAGT22850.026.4974179
AGTACGG18500.025.84759913
CGAACCT1509.094947E-1025.59281353
CGAACTA951.0686534E-525.36194224
Produced by FastQC (version 0.11.5)