FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112988
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63380.5694580714257477No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42030.3776321038501763No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39600.3557989843556265No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37440.33639176702713774No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36580.32866481938709136No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34890.3134804687921164No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC34870.31330077233537107No Hit
GAGTTACAATACCCCCGTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT34230.3075504857195226No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33400.30009308276459407No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32330.2904793223287223No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA31260.2808655618928506No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCAGAGCATTACCACCTATTTAAATTGGTATCAGCAGAAA30550.27448633767839364No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30410.2732284624811768No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT28480.2558877544052586No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCAGAGCATTACCAC28200.25337200401082494No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28200.25337200401082494No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC27600.24798111030846695No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC27480.24690293156799534No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26690.23980492152655733No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTC26660.23953537684143944No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA26100.234503876052572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25060.22515966030181817No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24760.2224642134506392No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24680.22174542762365812No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT24210.21752256089014438No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24140.21689362329153594No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA24110.21662407860641805No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23150.20799864868264525No Hit
ACTTACTACTGTCAACAGAGTTACAATACCCCCGTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA23040.2070103181705463No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22660.20359608549238625No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21880.1965879236793209No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA21010.18877112781090183No Hit
CACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACCCCCGTGACGTT20960.18832188666903865No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20800.18688431501507652No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20740.1863452256448407No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20660.18562643981785967No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20390.18320053765179858No Hit
GGGGTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTG20110.18068478725736487No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19950.17924721560340273No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19940.1791573673750301No Hit
AGTCAGAGCATTACCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATG19320.17358677721592686No Hit
GATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACC19120.1717898126484742No Hit
GTTACAATACCCCCGTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA19050.17116087504986577No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18900.1698131516242763No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC18660.16765679414333307No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18620.16729740122984255No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18290.16433240969354568No Hit
CTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGA18270.1641527132368004No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18190.16343392740981935No Hit
GTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCAGA18020.16190650752748456No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAAT18020.16190650752748456No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT17450.1567851585102445No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17440.15669531028187186No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17340.15579682799814554No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACA16960.1523825953199855No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT16570.1488785144134528No Hit
ATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAAT16250.14600337110552855No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAAGT16020.14393686185295798No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT15880.14267898665574114No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15500.13926475397758106No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG15450.1388155128357179No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15440.13872566460734526No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTA15310.13755763763850104No Hit
CAATACCCCCGTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT15300.1374677894101284No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15100.13567082484267576No Hit
CCCCTAAACTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCA15040.13513173547243995No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14960.13441294964545888No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTG14790.13288552976312415No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATT14340.12884235948635567No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCA14310.12857281480123775No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14090.1265961537770398No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14090.1265961537770398No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACCCCCGTGACGTTCGGCCAAGGGA14000.12578751972168611No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13970.12551797503656822No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13830.12426009983935137No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13590.12210374235840818No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT13490.12120526007468185No Hit
CCTTGGTCCCTTGGCCGAACGTCACGGGGGTATTGTAACTCTGTTGACAG13420.12057632247607342No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13250.11904890259373867No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCATCAT13200.11859966145187548No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13170.11833011676675759No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13120.11788087562489442No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12760.11464633940347965No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGGTA12750.114556491175107No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12730.11437679471836175No Hit
GTGGATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGGTAATG12720.1142869464899891No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12680.11392755357649857No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12600.11320876774951752No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12560.11284937483602697No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12550.11275952660765436No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC12530.11257983015090908No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCACGGG12520.11248998192253645No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA12500.1123102854657912No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12480.11213058900904592No Hit
GAGCATTACCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC12460.11195089255230065No Hit
CTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCT12430.11168134786718276No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12410.11150165141043748No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA12300.11051332089833851No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12210.10970468684298484No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12140.10907574924437641No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12100.10871635633088587No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12070.10844681164576797No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11970.10754832936204163No Hit
TCACGGGGGTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCA11850.10647015062157003No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11810.10611075770807953No Hit
CATCATCACTTGCCGGGCAAGTCAGAGCATTACCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC11620.10440364136899949No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCG11610.10431379314062685No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11580.10404424845550896No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11580.10404424845550896No Hit
ATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGGTAATGCT11550.10377470377039107No Hit
GGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC11450.10287622148666471No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT11390.10233713211642893No Hit
CAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA11260.10116910514758469No Hit
GTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCA11230.1008995604624668No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11190.10054016754897627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAC200.00752801151.4535831
GTGGTAT2200.046.775991
GTATCAA20800.043.3727341
TATCAAC23200.038.4371532
ATCAACG23950.037.090283
TCAACGC24150.036.925134
CAACGCA24050.036.7934465
AACGCAG24850.035.746976
AATGGTA10600.035.73639770
GTCAATC501.71763E-434.97498759
ACGCAGA26750.033.2258457
CGCAGAG27150.032.7392738
AGAGTAC27500.032.07588211
GCAGAGT28400.031.1788439
CTATAGT450.0047574930.4869044
TGTTAGA11400.030.1518670
CGGCGTA500.00738221727.86509754
ATAATCG500.00754640227.73980149
AGTACGG24900.027.70606413
GAGTACG24950.027.65054112
Produced by FastQC (version 0.11.5)