FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125067
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGAAGATACA62540.2942966033541531No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTTCTGATGGTGAAAGTAAAATCTGTC50210.23627490333245965No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47040.22135772660344358No Hit
GTATGATAATGCCCCCCTGATGTACACTTTTGGCCAGGGGACCAAGCTGG40270.18949990753232723No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG37050.17434744410411532No Hit
GTGTATTACTGTGCAAGGGGATATTGTACTAGGAACAGTTGCCACTTTGA34380.1617831343670576No Hit
GCATAAACCAGGTAAAGCCCCTAGGCTCCTGATCTACGATGCATCCAGTTTGGGAACAGGGGTCCCATCAAGGTT34140.1606537582109176No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32120.15114817556340576No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31470.14808944847385988No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG29750.1399955860215231No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28560.13439576258066216No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28430.13378401716275298No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28290.13312521440500463No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC28270.1330310997253263No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27870.1311488061317596No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27520.12950179923738875No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26310.12380786111684948No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26300.12376080377701033No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG26150.12305494367942282No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26050.12258437028103114No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT25920.12197262486312195No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGTAA25810.12145499412489112No Hit
GTGCAGCCACAGTTCGTTTGATCGCCAGCTTGGTCCCCTGGCCAAAAGTGTACATCAGGGGGGCATTATCATA25750.12117265008585612No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25470.11985504457035943No Hit
GTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGTAACCATTTAAATTGGTATCAGCATAAA24890.11712571885968773No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22830.10743190685281923No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21360.10051447789646162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)