FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125067
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGAAGATACA51850.2439923070660831No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45330.21331092149094594No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTTCTGATGGTGAAAGTAAAATCTGTC45000.21175802927625342No Hit
GTATGATAATGCCCCCCTGATGTACACTTTTGGCCAGGGGACCAAGCTGG38970.18338245335323544No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG37940.1785355473498012No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32660.15368927191472082No Hit
GCATAAACCAGGTAAAGCCCCTAGGCTCCTGATCTACGATGCATCCAGTTTGGGAACAGGGGTCCCATCAAGGTT30490.14347782916962148No Hit
GTGTATTACTGTGCAAGGGGATATTGTACTAGGAACAGTTGCCACTTTGA29290.1378309483889214No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29170.13726626031085137No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28990.13641922819374636No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28720.13514868001808883No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27970.1316193795301513No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27360.12874888179996208No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27200.1279959643625354No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26470.12456077855427616No Hit
GTGCAGCCACAGTTCGTTTGATCGCCAGCTTGGTCCCCTGGCCAAAAGTGTACATCAGGGGGGCATTATCATA26390.12418431983556283No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGTAA26180.12319611569894032No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25800.12140793678505195No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25410.11957270053132442No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25250.11881978309389776No Hit
GTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGTAACCATTTAAATTGGTATCAGCATAAA23740.11171412477818346No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23430.11025534724316928No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23270.1095024298057426No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT23240.1093612577862251No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCATTAACTACGAAATGCACTGGGTCCGCCAAGCTACAGGAAAAGGT22720.10691427611458838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)