FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463508
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCCTTCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT88310.3584725521492116No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT86420.3508005656973714No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT78230.3175552910727304No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT78160.317271143426366No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC76330.309842712099981No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA68810.279317136376257No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC64960.26368901582621207No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG63580.2580872479407414No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT63460.2576001376898309No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG62370.2531755529107273No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGCCGCTGATGGTGAGAGTGAACT61500.24964400359162625No Hit
CCTCATGTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGAGTT56180.22804878246792784No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55400.22488256583700966No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53060.2153839159442551No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT52450.21290777216879342No Hit
TTCCTGTACAGCCTCTGGATTTAGTTTTAGTAGCTATTGGATGAGCTGGGTCCGGCAGGCTCCAGGGAAGGGG52340.21246125443879216No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCGGCCTGCAGTCTGAAGATT51310.2082802247851438No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA50250.20397741756876778No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC49830.20227253169058107No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49530.20105475606330483No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT48510.19691431893056566No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA47270.19188084633782396No Hit
GAGTTCACTCTCACCATCAGCGGCCTGCAGTCTGAAGATTCTGCAGTTTATTACTGTCAGCAGTATAATAACTGG47020.19086603331509375No Hit
CTCCAGGGAAGGGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGT46820.1900541828969096No Hit
GTGAAGGGCCGATTCACCATCTCCAGAGACAACGGCAAGAACTCACTGTA46720.18964825768781754No Hit
ACCCTGGGGGGTCCCTGAGACTTTCCTGTACAGCCTCTGGATTTAGTTTT46640.18932351752054388No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC46380.18826811197690446No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT45980.18664441114053618No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAG45690.18546722803416915No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG45640.18526426542962313No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAACAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT45270.18376234215598244No Hit
CTGATATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGAGTTCACT44820.1819356787150681No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC44330.17994664519051692No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44110.17905360973051437No Hit
GTCCCAGACCCACTGCCACCGAACCTGGCTGGGATATCAGTGGCCCTGGT43460.17641509587141588No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG43100.1749537651186844No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT42660.17316769419867928No Hit
CTCCAGCCCCTTCCCTGGAGCCTGCCGGACCCAGCTCATCCAATAGCTAC42440.1722746587386767No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG42030.1706103653813992No Hit
GAGTATTAACAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTGCATC41840.16983910748412426No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41450.16825599916866518No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA38680.1570118708768147No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA38540.15644357558408578No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37800.15343972903680445No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGTGAGACATACTA37560.15246550853498345No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATGAAGGGCCAGTTATTATACTG37240.15116654786588882No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT36730.14909632929951921No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA36640.14873099661133635No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT36580.1484874414858811No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC36540.14832507140224427No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG36220.14702611073314964No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35450.14390048662314064No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC35400.14369752401859462No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAGCAACCTAGCCTGGTA34820.1413431578058606No Hit
ATACATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAGGTTGCTGTTAATACTCTGACTGGC34590.1404095298249488No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG34530.14016597469949357No Hit
GCCCTGGACTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG33590.13635027773402805No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG33530.13610672260857282No Hit
CTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC33490.135944352524936No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT33460.13582257496220837No Hit
GCCTTAGCCCTGGACTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGC33270.13505131706493342No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC32940.13371176387492958No Hit
CTCCAGAGACAACGGCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACTCGTCCGTTTATTT32790.13310287606129145No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC32730.13285932093583622No Hit
CGTCCATACCGTAGTAGTAGGAGTTTTGCCTAACGAGGGAGCAGGTACCA32690.1326969508521994No Hit
ACATAAAGCAAGATGGAAGTGAGACATACTATGTGGGCGCTGTGAAGGGC32500.13192569295492446No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT31650.1284753286776418No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG31460.12770407078036686No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30530.12392896633581056No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCA30350.12319830095944481No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACCGAACCTGGCTGGGATATCA30340.12315770843853562No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30150.12238645054126068No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA29970.12165578516489493No Hit
TCCCTGGAGCCTGCCGGACCCAGCTCATCCAATAGCTACTAAAACTAAAT29870.12124985995580287No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA29770.12084393474671078No Hit
GAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT29680.12047860205852791No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC29670.12043800953761871No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG29630.1202756394539819No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA29200.11853016105488597No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29110.11816482836670308No Hit
CATGTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGAGTTCA28940.11747475551124656No Hit
GTGTGAGGTGCAGCTGGTGGAGTCGGGGGGAGGCTTGGTCCACCCTGGGGGGTCCCTGAGACTTTCCTGTACAGC28850.1171094228230637No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACC28740.1166629050930624No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28730.1166223125721532No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT28720.116581720051244No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT28250.11467387156851125No Hit
GTATTAACAGCAACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTGCATCCA28060.1139026136712363No Hit
GGTCCACCCTGGGGGGTCCCTGAGACTTTCCTGTACAGCCTCTGGATTTA27890.11321254081577978No Hit
CAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGC27850.11305017073214293No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT27680.11236009787668642No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC27510.11167002502122989No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC27270.1106958045194089No Hit
CCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGAGTTTTGCCTAACGAGGGAGCAGGTACCAAAACTGCAAC27250.11061461947759049No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAGCTCATC27190.11037106435213524No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG27120.1100869167057708No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27060.10984336158031555No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT26850.10899091864122218No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG26750.10858499343213011No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC26730.1085038083903117No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC26680.10830084578576567No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26630.10809788318121961No Hit
GCCCTTCACAGCGCCCACATAGTATGTCTCACTTCCATCTTGCTTTATGTTGGCCACCCACTCCAGCCCCTTCC26430.10728603276303549No Hit
GCCTGAGAGCCGAGGACTCGTCCGTTTATTTCTGTGCGAGAGGTTGCAGT26420.10724544024212627No Hit
GTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCGGTGGCAGTGGG26000.10554055436393955No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA25980.10545936932212113No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25960.10537818428030271No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25940.1052969992384843No Hit
CACCAGGGCCACTGATATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCGG25840.10489107402939224No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC25810.10476929646666461No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25540.103673298402116No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA25380.10302381806756868No Hit
CCGTAGTAGTAGGAGTTTTGCCTAACGAGGGAGCAGGTACCAAAACTGCA25210.10233374521211216No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24980.10140011723120039No Hit
GGATATCAGTGGCCCTGGTGGATGCACCATACATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG24950.10127833966847276No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG24780.10058826681301623No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA24770.10054767429210704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)