FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829506
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15580.18782263178325412No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCA14230.1715478851268104No Hit
GTATTATTGTCAGCAGTATGGTAGGACGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG11130.13417624465645817No Hit
GTATGGTAGGACGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT10720.12923354382005675No Hit
CTACCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC9340.1125971361268032No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9140.11018606254807078No Hit
ATGGTAGGACGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA9140.11018606254807078No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8950.10789554264827499No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG8740.10536391539060597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8420.10150619766463413No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8340.10054176823314116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17550.040.737231
ATCAACG19600.035.225953
TATCAAC19700.035.047142
CAACGCA19700.035.047145
TCAACGC19850.034.6083874
AACGCAG20350.033.7580576
GTGGTAT3650.033.118711
TGGTATC3850.031.3831182
AGAGTAC22550.030.77447511
ACGCAGA22800.030.431537
CGCAGAG23350.029.4208348
CAGAGTA24250.028.6170910
GGTATCA8850.028.0987821
TTATACG756.0752624E-527.65715635
CTAGTAC500.00771029727.6171463
GCAGAGT25950.026.7423659
CGTAACA1057.1383147E-726.50656553
TTACGCA700.001328717724.6581634
AGTACGG21000.024.49672913
GAGTACG21600.023.97610712
Produced by FastQC (version 0.11.5)