FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543226
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43200.2799330752592297No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40230.26068767633515766No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31660.20515465654414844No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30740.19919311883029445No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29870.19355557773132387No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27960.18117890704277922No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27020.1750877706829719No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24560.15914713723071022No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCGCACCTCTTTAAATTGGTATCAACAAAAA24380.15798074941713008No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24340.1577215521252234No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTCCCAGATCCACGTCCA23390.1515656164424394No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22450.1454744800826321No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22180.1437248983622619No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22160.14359529971630858No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCCGCCGAAAATG21650.14029053424449822No Hit
ATATTACTGTCAACAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGA21360.1384113538781747No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21060.13646737418887447No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20430.13238501684134404No Hit
AGTCAGGACATTCGCACCTCTTTAAATTGGTATCAACAAAAACCAGGGAA20420.13232021751836737No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19780.12817306084786026No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTCCCAGATCCACGTCCA19610.1270714723572568No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCGCAC19360.12545148928283997No Hit
ACATATTACTGTCAACAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAA19230.12460909808414322No Hit
CAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCAT19180.12428510146925986No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19040.1233779109475864No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT18580.12039714209065944No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18520.1200083461527994No Hit
ATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCAT18480.11974914886089269No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCCGCCGAAAATGAGGGGGAGA18210.11799956714052251No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18210.11799956714052251No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18130.11748117255670912No Hit
GCCATATTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGC18080.11715717594182576No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC17460.113139617917272No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG16600.10756687614127808No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT16090.10426211066946772No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15830.10257732827207422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC38900.035.819314
ATCAACG39150.035.6784553
CAACGCA39450.035.3199275
AACGCAG40100.034.747416
ATCGCGT501.8495454E-434.5385137
ACGCAGA44150.031.5599357
AGAGTAC44100.031.44376411
GTATACG553.298514E-431.296851
CGCAGAG44750.031.0619168
CAGAGTA45150.030.71251310
GTGGTAT5450.028.425581
GCAGAGT49100.028.380079
GTATCAA52800.026.7979281
TTATACA3250.026.4647882
CTTATAC3200.025.81991
TATGCCG2150.025.79200643
CGACCGG1501.1275634E-425.2536370
TCGTATG1651.10958354E-1025.16557540
AGTACGG44600.024.76499613
CGTATGC2250.024.61596541
Produced by FastQC (version 0.11.5)