FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543226
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42660.2764339118184893No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35520.23016719521314438No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33100.21448575905278938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31410.2035346734697316No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28730.18616845491198308No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25590.16582146749730758No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25250.1636182905161007No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25170.1630998959322873No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24560.15914713723071022No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24530.1589527392617802No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCGCACCTCTTTAAATTGGTATCAACAAAAA22780.14761285774086233No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCCGCCGAAAATG22670.1469000651881189No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22620.14657606857323555No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22010.14262330987165847No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20990.13601377892803776No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20820.1349121904374343No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20380.13206102022646068No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTCCCAGATCCACGTCCA20190.13082983308990387No Hit
AGTCAGGACATTCGCACCTCTTTAAATTGGTATCAACAAAAACCAGGGAA20030.1297930439222771No Hit
ATATTACTGTCAACAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGA19850.12862665610869697No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19750.12797866287893025No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18880.12234112177995965No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCGCAC18720.12130433261233287No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18320.11871235969326593No Hit
ACATATTACTGTCAACAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAA18270.11838836307838255No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18240.11819396510945254No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTCCCAGATCCACGTCCA17870.11579639015931562No Hit
ATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCAT17790.11527799557550222No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG16900.10951085583057829No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16810.10892766192378822No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT16690.10815007004806815No Hit
GCCATATTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGC16600.10756687614127808No Hit
CAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCAT16470.10672448494258133No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCCGCCGAAAATGAGGGGGAGA16460.10665968561960465No Hit
GGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG16340.10588209374388455No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15910.1030957228558876No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC15830.10257732827207422No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15740.10199413436528415No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15650.10141094045849408No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15470.10024455264491397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)