FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461726
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG352.2594424E-549.32728640
TGAATCG751.8189894E-1145.9089634
TATACAC1650.043.7513
GTATCAA11350.043.660611
TATCAAC12400.039.3658182
TTATACA1850.039.0211642
ACGAGAC2000.036.09848422
TCAACGC13650.035.7608874
CAACGCA13750.035.500815
ATGAATC1850.035.362333
ATCAACG14000.034.8668673
AATCGCG501.8730889E-434.45788236
ATATCTG400.002641033134.3757863
AACGCAG14300.034.1353956
TACACAT2300.032.8811845
AGCGAAT800.003433941432.5765670
GGTATCA6150.031.8950251
TCTCGTA652.3189416E-531.84530338
ACGCAGA15450.031.3720747
CTCGTAT553.2503786E-431.36961739
Produced by FastQC (version 0.11.5)