FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249751
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGCGTCCGCGGCGGTCACGGAGGTCAGCTTCAGGGAGAACTGGTTGTTGGACGTGTCTAACGA44670.3574312002950988No Hit
GTGTATTACTGTGCGAAATCGGAAATTGCCCTCCGTACTGGGAGCTACTA29160.233326478634544No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGCGTGCTTGTATATGAA26870.21500282856344982No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGC26590.2127623822665475No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24080.19267838153360148No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23450.1876373773655712No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG22290.1783555284212615No Hit
TCTCAAGACTCCGGGCTGAGGACGAGGCTGATTATTCCTGCAGTTCATAT22150.17723530527281034No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21800.1744347474016824No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21590.17275441267900565No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21550.17243434892230533No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21390.17115409389550398No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21290.17035393450375316No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20080.160672005863568No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT19610.1569112567223391No Hit
CACCTGCACTGTCGCTGGTGACTCCATCAGCAGTAGTGGTTACTACTGGG19450.15563100169553773No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC19190.15355058727698556No Hit
GTCTTGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG18640.14914971062235596No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18600.14882964686565564No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18430.1474693758996792No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGCGT18350.14682924838627856No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18030.14426873833267587No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16880.13506690532754123No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16070.12858561425435946No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15840.12674524765333256No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15720.12578505638323156No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15580.12466483323478038No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCTGGTACCAACAACA15220.12178425942447736No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCTGGTACCAACAACACCCAGGCAATGC14940.119543813127575No Hit
CTACTGGGGCTGGCTCCGCCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGAGTATCTTTTACGATGGGAGCCC14890.1191437334316996No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14790.11834357403994876No Hit
GTTCATATACAAGCACGCGCACGCTCGTGGCTTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGG14650.11722335089149759No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14540.11634317556057167No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14410.11530296835129557No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13910.11130217139254138No Hit
GTATATGAACTGCAGGAATAATCAGCCTCGTCCTCAGCCCGGAGTCTTGA13750.11002191636574005No Hit
GTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTC13210.10570105565028555No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13180.1054610078327603No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13110.10490089625853471No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13060.10450081656265928No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12950.10362064123173335No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12840.10274046590080745No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12820.10258043402245727No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12680.10146021087400611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)