FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191598
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27130.22767745498062267No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24150.20266902092819897No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23700.19889257954444367No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21790.18286368389339358No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21090.17698921951866317No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20880.17522688020624405No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19780.16599557904595344No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18720.1570999615642188No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18530.15550546409107768No Hit
TCTATGCCCCGTCCCTGAAGACACGAGCCACCATATCCGTAGACACGTCC17820.14954707879670828No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT17020.14283340522558782No Hit
GAAATACACAGCCGTGTCCGCGGCGGTCACAGAGTTCAGCCTCAGGGAAA16110.1351966015384383No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15950.13385386682421418No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15830.13284681578854615No Hit
CTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCCCCA15820.13276289486890713No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15580.13074879279757098No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15550.13049703003865398No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15390.12915429532442987No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14950.12546177486031362No Hit
GCATTATAGTGGGAGCGCCTTCTATGCCCCGTCCCTGAAGACACGAGCCACCATATCCGTAGACACGTCCAAGAA14820.12437080290500656No Hit
CTATAATGCATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG14680.12319591003006047No Hit
GTGCAGCCACAGTTCGTTTGATATCGACTTTGGTCCCAGGGCCGAAAGTG14580.12235670083367042No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14330.12025867784269527No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14140.11866418036955416No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13860.11631439461966199No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13840.116146552780384No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13600.11413245070904786No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13580.11396460886976983No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA13390.11237011139662872No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13150.11035600932529259No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12850.10783838173612241No Hit
ATGTAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA12670.10632780518262032No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12530.10515291230767425No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12390.10397801943272816No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAGCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA12360.10372625667381112No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTAATCGGGCC11990.10062118264716792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2300.050.9835781
GGTATCA8000.040.524451
TCGTATG459.8819844E-538.4210340
TGGTATC3050.038.4159772
GTATCAA17450.035.773581
CGTATGC501.8325413E-434.59203341
GTACTAG553.2608426E-431.35748930
ACCGTAC553.2648607E-431.35090427
TAACGGC802.7416208E-630.21584936
TATCAAC21400.029.3082892
GTTATCG605.2352983E-428.92293749
TCAACGC21500.028.85144
ATCAACG21750.028.5197733
CAACGCA22000.028.3523275
GTATATA500.00774719327.5911121
AACGCAG22650.027.5386876
GTTATTA950.00802078727.39358770
CGCGGTA906.7207293E-626.92759943
TACTAGA658.6565677E-426.53883631
ACGAGAC658.701259E-426.51544222
Produced by FastQC (version 0.11.5)