FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191598
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28270.23724443981946933No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24160.20275294184783793No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20460.1717022015814058No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20410.17128259698321077No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19640.16482068617100734No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19600.16448500249245132No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17340.145518874654036No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT15630.13116839739576602No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15630.13116839739576602No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15590.13083271371721No Hit
GAAATACACAGCCGTGTCCGCGGCGGTCACAGAGTTCAGCCTCAGGGAAA15130.12697235141381574No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15120.12688843049417672No Hit
GTGCAGCCACAGTTCGTTTGATATCGACTTTGGTCCCAGGGCCGAAAGTG14950.12546177486031362No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14520.12185317531583637No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14450.12126572887836333No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14450.12126572887836333No Hit
TCTATGCCCCGTCCCTGAAGACACGAGCCACCATATCCGTAGACACGTCC14420.12101396611944631No Hit
CTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCCCCA14240.11950338956594421No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14210.1192516268070272No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14010.11757320841424708No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13990.11740536657496908No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13700.11497165990543791No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13550.11371284611085282No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13510.11337716243229679No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13510.11337716243229679No Hit
CTATAATGCATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG13480.11312539967337977No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13350.1120344277180727No Hit
GCATTATAGTGGGAGCGCCTTCTATGCCCCGTCCCTGAAGACACGAGCCACCATATCCGTAGACACGTCCAAGAA13070.10968464196818055No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13060.10960072104854153No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA12630.1059921215040643No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12510.10498507046839622No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12500.1049011495487572No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12400.10406194035236715No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA12240.10271920563814307No Hit
GTGTATTTCTGTGCGAGACGGCCTACCAGCAACAGCTACTACGAGTACTT12110.101628233682836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)