FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180505
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14190.12020279456673204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13100.042.5034981
GTATCAA31600.039.8084221
GTGGTAT4650.039.1745381
ATCAACG36750.033.848563
TCAACGC37300.033.4415784
CAACGCA37850.033.1386155
TATCAAC37700.032.9044652
AACGCAG39300.032.1809926
ACGCAGA41700.030.4241527
AGAGTAC41750.030.15088511
CGCAGAG41950.030.0891238
CAGAGTA42750.029.68695410
TGGTATC6000.028.6356932
GCAGAGT47100.026.9451669
AGTACGG34900.025.81970613
GAGTACG36250.024.95302212
GTACTTT15050.023.53835714
GAGTACT15100.023.46041512
AGTACTT15250.023.2296613
TACGGGT4450.023.18854515
Produced by FastQC (version 0.11.5)