FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359067
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTACTACAGTCCGTCCCTCAAGAGTCGAGTCTCCATATCGGTTGACACGTCCAGGAACCAGTTCTCCCTGAGG25560.18807019815800105No Hit
GTAGTAGGTACTCCCTTCATAAGAGATACTCCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAGCC23040.16952806594524036No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18780.13818303291890688No Hit
GTAAAAGACAGCCGTGTCAGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA18730.137815133470241No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18080.13303244063758446No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18070.1329588607478513No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16440.12096533872134338No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16080.118316462690949No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15650.1151525274324224No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13630.10028938970632058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.038.698661
GTATCAA21600.037.565781
TATCAAC24500.032.9600832
ATTAGAC450.004616767630.6751063
TCAACGC26300.030.5730484
CAACGCA26900.029.891125
ATCAACG27100.029.6705253
AACCGAT7750.029.40226770
AACGCAG27500.029.238956
GTGGTAT5800.027.9800321
GTAAGTT950.00799436527.41263870
AGAGTAC30150.026.3266211
CGCAGAG31300.025.6891758
ACGCAGA32350.024.9611247
CAGAGTA32200.024.86489910
TTTAACG851.430039E-424.38655934
GCAGAGT33550.024.0700999
TGGTATC6750.024.0288312
TCTTATA1301.843E-723.8911882
AGTACGG31000.023.04428313
Produced by FastQC (version 0.11.5)