FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209743
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAACGGCCATTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTC73810.6101295895078541No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC63660.5262274714546809No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC56000.46290823753474913No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGG47190.3900828523083002No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46220.3820646203367161No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG42170.3485864353007209No Hit
CCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGT41020.33908028399420376No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT40730.3366830806212559No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39350.32527569905343534No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT38740.3202333057517175No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG38380.31725746708185126No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC37170.3072553426636897No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG36550.3021302871766979No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC36030.2978318535424466No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGT34530.28543252575133726No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32940.27228923829276136No Hit
CCCTTGAGGGCCGCTGATTATCACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTTAA32420.2679908046585101No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31420.2597245861311039No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCA31380.2593939373900076No Hit
GCCATTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC31240.25823666679617074No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30500.2521196650858901No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30210.24972246171294232No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC29520.24401877092903204No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGA29390.24294416252046924No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29100.24054695914752142No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG28940.23922436418313642No Hit
GCATGGGATGACAGCCTGAACGGCCATTGGGCGTTCGGCGGAGGGACCAA27280.2255024414276421No Hit
CCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT27140.22434517083380517No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26770.22128666997866486No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCAATGGCCGTTC26690.22062537249647238No Hit
CTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTT25470.21054058589303676No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25340.20946597748447396No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24970.20640747662933367No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24510.20260501610672682No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24110.1992985286957643No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG23950.1979759337313793No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23930.1978106093608312No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT23000.19012302613034338No Hit
GTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTTAACAGTATTTCTT22760.1881391336837659No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21880.1808648613796484No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA21870.18078219919437433No Hit
CACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21260.17573980589265653No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21120.17458253529881967No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20760.17160669662895342No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC20760.17160669662895342No Hit
GGATGACAGCCTGAACGGCCATTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC20760.17160669662895342No Hit
CTCTTGTTCTGGAAGCACCTCCAACATCGGAAGAAATACTGTTAACTGGTACCAGCAGTTCCCAGGAACGGCCCC20310.16788689829162062No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG20250.16739092517997622No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20140.16648164114196157No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT19940.1648283974364803No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGA19870.16424976213956188No Hit
GTGATAATCAGCGGCCCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAG19660.16251385624880657No Hit
CATTTATAGTGATAATCAGCGGCCCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC19570.16176989658134No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG19170.15846340917037752No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19030.15730613857654063No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG18760.15507425957414095No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC18590.1536690024244819No Hit
GTGCAGCATGGGATGACAGCCTGAACGGCCATTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGC18460.1525943940159191No Hit
GATTATCACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGG18440.15242906964537095No Hit
CCCAATGGCCGTTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC18280.15110647468098595No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18010.1488745956785863No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18010.1488745956785863No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18010.1488745956785863No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA17740.1466427166761866No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17580.1453201217118016No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17450.1442455133032388No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCTTGAGGGCCGCTG17180.1420136343008391No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCA17110.14143499900392068No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT17020.1406910393364541No Hit
GTCAGGGACCCTTGAGGGCCGCTGATTATCACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTA16870.13945110655734316No Hit
GTATTTCTTCCGATGTTGGAGGTGCTTCCAGAACAAGAGATGGTGACCCT16710.1381285115929582No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16530.13664059225802505No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGC16380.1354006594789141No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC16270.13449137544089942No Hit
GCCCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG16080.13292079392069225No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16040.132590145179596No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15780.13044092836247037No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG15750.1301929418066482No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15530.12837437373061883No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15510.12820904936007071No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGACTTGAAG15380.1271344409515079No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCC15310.12655580565458943No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA15280.12630781909876726No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC15030.1242412644669157No Hit
GGGTCACCATCTCTTGTTCTGGAAGCACCTCCAACATCGGAAGAAATACT14740.12184406109396789No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAACGGCCATTGGGCGTTCGGCG14650.12110010142650134No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14550.12027347957376071No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC14460.11952951990629415No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC14430.11928153335047195No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14170.11713231653334634No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC14100.1165536812364279No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13990.11564439719841321No Hit
GAAGAAATACTGTTAACTGGTACCAGCAGTTCCCAGGAACGGCCCCCAAA13990.11564439719841321No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAC13890.11481777534567258No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCACCTCCAACATCGGAAGAAATACTGTTAA13880.11473511316039853No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG13680.11308186945491727No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG13680.11308186945491727No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC13600.11242057197272479No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG13570.11217258541690259No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13490.11151128793471009No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCAATGGCCGTTCA13480.11142862574943604No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13390.11068466608196949No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC13210.10919674674703635No Hit
GTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13030.10770882741210322No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG13030.10770882741210322No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG12930.10688220555936262No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12490.10324506940730387No Hit
TGGGATGACAGCCTGAACGGCCATTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG12460.10299708285148167No Hit
AGACACACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC12410.10258377192511137No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT12210.10093052821963011No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12200.10084786603435605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20300.043.242661
GGTATCA7100.042.1821941
TTCGACG350.00136187639.34559618
CGATTGT859.133364E-538.93486470
TATCAAC23050.037.7754942
ATCAACG24700.035.2520263
TCAACGC25050.034.7594874
CAACGCA25600.034.01275
AACGCAG25750.033.820166
TCCGAGA1601.02116246E-733.09463570
ACGCAGA27250.031.9664277
GTCTAGG553.2992105E-431.2950461
CGCAGAG28050.030.9345428
AGAGTAC28200.030.7725411
TCGACGA450.00466594830.60845619
GAGTAAA450.004672559430.59961
CAGAGTA28850.030.19858710
TGGTATC2850.030.1894862
GTGGTAT3100.029.9826721
TACATAC703.915759E-529.499443
Produced by FastQC (version 0.11.5)