FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859262
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27680.32213690352884217No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12520.1457064317984503No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10010.11649531807527856No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9180.10683586612697873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8000.042.987341
GTATCAA18200.038.92481
CGACCGG700.002027585636.27877470
TATCAAC20400.034.8854522
ATCAACG22450.031.5467833
TCAACGC22800.031.3640884
TTTAGCG553.2942582E-431.3017826
CAACGCA23000.030.941885
AACGCAG23550.030.2192446
ACGATTG703.639892E-529.8154547
ACGCAGA25100.028.3630187
AGAGTAC25250.028.19945111
CAGAGTA25850.027.5449210
CTCGCTA500.00782648327.53273223
CGCAGTA756.294863E-527.513518
CGCAGAG25850.027.2771978
TATACTG1608.185452E-1125.78495
ATGCGTT700.001196302925.10636361
GCAGAGT28550.024.6990059
TAATATC700.001360953724.5570474
Produced by FastQC (version 0.11.5)