FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484248
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59500.4008764034042828No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG55510.37399410341128975No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43000.2897089974182212No Hit
AGTCAGGACATCACCAATTTTTTAAGTTGGTTTCAACAGAAACCAGGGGG41990.2829042046881653No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41190.2775142698524775No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG38120.2568303949205254No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37680.2538659307608971No Hit
GTAGTATGTTGCAATATCGTCAGGCTGCAGGTTGGTGATGGTGAGAAAAAAATTTGTCCCGGATTTACGTCCA37170.2504298473031461No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36580.2464547703618263No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCAGGCGAGTCAGGACATCACCAATTTTTTAAGTTGGTTTCAACAGAAA36510.24598315106370366No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33710.2271183791387962No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTT33640.2266467598406735No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33390.22496240520452104No Hit
GTTTAGGGGCTCCCCCTGGTTTCTGTTGAAACCAACTTAAAAAATTGGTG32010.21566476761295958No Hit
GTGCAGCCACAGTTCGTCTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTT31930.2151257741293908No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31850.21458678064582198No Hit
CCCCTAAACTCCTCATCTACGATGCATCCAATTGGGGTCCTGGAGTCCCA31590.21283505182422344No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCT30390.2047501495706917No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30070.20259417563641657No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAGCTCCACCTTGGTCC26940.1815060555917879No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC26410.1779352237631447No Hit
ACATACTACTGTCAACAATATAGTGATTTCCCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGCTCAGACGAA26240.17678986261056104No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25780.17369065008004053No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT25600.17247791474201077No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25580.17234316637111857No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24570.16553837364106266No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23750.16001369043448266No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23510.1583967099837763No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT23030.1551627490823636No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCAGGCGAGTCAGG23000.1549606265260253No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22870.154084762115226No Hit
ACCTTGGTCCCTCCGCCGAAAGTTCGAGGGAAATCACTATATTGTTGACAGTAGTATGTTGCAATATCGTCAGG22240.14984018843212185No Hit
CAGTAGTATGTTGCAATATCGTCAGGCTGCAGGTTGGTGATGGTGAGAAAAAAATTTGTCCCGGATTTACGTCCA21610.14559561474901767No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21600.14552824056357158No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21580.14539349219267939No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21390.14411338266920354No Hit
TCACTATATTGTTGACAGTAGTATGTTGCAATATCGTCAGGCTGCAGGTTGGTGATGGTGAGAAAAAAATTTGTC21100.14215953129126668No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21020.1416205378076979No Hit
GACCAAGGTGGAGCTCAGACGAACTGTGGCTGCACCATCTGTCTTCATCT20620.13892557038985398No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGCCATTCAGATGACCCAGTCTCCATCCTCC20120.1355568611175491No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGCCATTCAGATGACCCAGTCTCCATC19750.13306401625604347No Hit
ATACTACTGTCAACAATATAGTGATTTCCCTCGAACTTTCGGCGGAGGGA19610.13212077765979807No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19180.12922368768561587No Hit
ATATAGTGATTTCCCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGCTCAGACGAACTGTGGCTGCACCAT18930.12753933304946344No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18680.12585497841331098No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18580.12518123655885No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18230.12282314006823658No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18110.1220146498428834No Hit
GCCACAGTTCGTCTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTTCGAGG18060.1216777789156529No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17690.11918493405414729No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17560.118309069643348No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGCCATTCAGATGACCCAGTCT17230.11608572152362678No Hit
ATATTGCAACATACTACTGTCAACAATATAGTGATTTCCCTCGAACTTTC17040.11480561200015091No Hit
GGTTGGTGATGGTGAGAAAAAAATTTGTCCCGGATTTACGTCCACTGAAT17010.11460348944381261No Hit
CAATTGGGGTCCTGGAGTCCCATCCAGATTCAGTGGACGTAAATCCGGGACAAATTTTTTTCTCACCATCACCAA16830.11339075410578286No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15850.10678808393206526No Hit
CTATATTGTTGACAGTAGTATGTTGCAATATCGTCAGGCTGCAGGTTGGT15640.1053732260376972No Hit
GTCCTGACTCGCCTGGCAAGAGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT15620.10523847766680501No Hit
GCTCTGGCTCTCAGGTGCCAGATGTGCCATTCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGA15520.10456473581234402No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCA15510.10449736162689792No Hit
CCCCAATTGGATGCATCGTAGATGAGGAGTTTAGGGGCTCCCCCTGGTTT15400.10375624558699086No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15330.10328462628886817No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15210.102476136063515No Hit
TCACCAACCTGCAGCCTGACGATATTGCAACATACTACTGTCAACAATAT15180.1022740135071767No Hit
GGATGGGACTCCAGGACCCCAATTGGATGCATCGTAGATGAGGAGTTTAGGGGCTCCCCCTGGTTTCTGTTGAAA15070.10153289746726964No Hit
GTAATAGACAGCCGTGTCTTCGACCCGCAGGTTAGTCAATAGGAAGGAAG14910.10045491050013206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.044.013241
GTATCAA31350.042.9602931
GTGGTAT3850.042.94461
TATCAAC35450.037.778252
TGGTATC4300.037.630322
ATCAACG38000.035.1525343
TCAACGC38000.035.0619354
CAACGCA38450.034.830665
TCGTATG905.0677045E-934.586240
AACGCAG39750.033.6915476
AGAGTAC41150.032.29648211
CGCAGAG42300.031.4998428
CAGAGTA42300.031.49984210
GCAGAGT43950.030.3955929
TTAACGG1052.3213943E-829.58523835
AGTACGG35450.028.84549513
CGTATGC1202.6011548E-928.83647241
ACGCAGA46450.028.7577257
GTATTAG2050.028.5642641
GAGTACG36050.028.55641612
Produced by FastQC (version 0.11.5)