FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757771
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT1001.565113E-743.54805470
GTATCAA17550.035.693851
TATCAAC18550.033.9283142
GGTATCA8500.033.609211
TCAACGC20050.031.3900384
ATCAACG20300.031.003463
CAACGCA20600.030.8858555
AACGCAG21400.029.7312456
ACGCAGA21650.029.3898687
AGAGTAC22350.028.77905511
CAGAGTA23250.027.9609110
CGCAGAG23100.027.844678
GCAGAGT25200.025.797279
GAGTACG19500.025.04760412
AGTACGG19550.024.98354513
TACGGGA8600.021.99476815
GTACGGG21850.021.87999214
ATAATAC953.1300375E-421.721153
ACTCGGT800.002950699821.49630522
GAGTACT7400.020.91670212
Produced by FastQC (version 0.11.5)