FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208610
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28850.041.50691
GGTATCA11350.040.4382551
ATCAACG32900.036.6801643
TCAACGC33800.035.6014674
CAACGCA33900.035.5981565
TATCAAC34550.034.629052
AACGCAG35700.033.9964526
GTGGTAT4500.032.2087141
ACGCAGA38250.031.7300227
CGCAGAG38550.031.5725368
AGAGTAC39000.031.1198311
CAGAGTA39800.030.58093810
GCAGAGT42800.028.6790929
AGTACGG33850.028.41873213
GAGTACG34550.027.74315812
TGGTATC4900.027.4427532
TACGGGA13650.025.512215
GACCGGA2251.3329736E-724.70168970
GTACGGG39250.024.33320214
TACGGGG17750.022.72723815
Produced by FastQC (version 0.11.5)