FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208610
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28100.041.409231
GGTATCA11400.038.653771
GTGGTAT4450.037.133681
ATCAACG33650.034.4700363
TCAACGC33600.034.214024
GACCGGA2053.274181E-1133.9693270
CAACGCA34100.033.7123455
TATCAAC34350.033.5671922
TACCGTG751.694254E-632.1389277
AACGCAG36050.031.9855926
ACGCAGA38350.030.0797397
AGAGTAC39500.029.3844311
CGCAGAG39600.029.0481038
CAGAGTA40150.028.7371510
CTATACG500.00782442227.5351144
GCAGAGT43750.026.3724969
TACCACG809.1567745E-526.04040145
TGGTATC5950.026.0310942
AGTACGG34500.025.3570713
GAGTACG34550.025.2206912
Produced by FastQC (version 0.11.5)