FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421430
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC13790.3272192297653228No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT13040.3094226799231189No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA10540.25010084711577246No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT9600.22779583798021022No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9260.2197280687184111No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA7910.18769427900244406No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC6770.16064352324229408No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6360.15091474266188928No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT6320.14996559333697174No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA6090.1445079847186959No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT5670.13454191680706168No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC5490.13027074484493273No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA5440.1290843081887858No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT5440.1290843081887858No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT5070.12030467693329852No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGA4900.11627079230239898No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTA4820.1143724936525639No Hit
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGC4790.11366063165887573No Hit
GGCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAG4640.11010132169043493No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT4620.10962674702797617No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4430.10511828773461784No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT4350.10321998908478278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1700.054.5673871
TGGTATC1900.048.817662
GGTATCA5900.044.256791
GTATCAA15350.040.736281
GTAGTTT4550.039.7104970
ATCAACG18700.032.8835143
TCAACGC18700.032.8835144
GCCTAAT652.3102448E-531.86183535
CAACGCA19300.031.8612295
AACGCAG19600.031.5488266
AGGATAC450.004368734531.0182850
TAGTTAG850.00574862329.31971270
TTTCGTT604.9323525E-429.21343655
CGCAGAG21300.028.88678
GCGGGTA605.534065E-428.6446369
AGAGTAC21700.028.19582411
ACTTATC500.007031400728.140359
CACGTCA905.0543695E-627.92520167
CAGAGTA22050.027.90416110
GCAGAGT22050.027.7482729
Produced by FastQC (version 0.11.5)