FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664168
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16900.10155224712889564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53250.041.5094151
GGTATCA21300.035.82851
ATCAACG61750.035.5696833
TCAACGC61700.035.4313774
TATCAAC62000.035.3708152
GTGGTAT8100.034.375991
CAACGCA63450.034.3458065
AACGCAG65000.033.6376086
ACGCAGA67950.032.1937037
CGCAGAG68700.031.8951858
AGAGTAC70250.031.2912611
CAGAGTA73050.030.04477710
GCAGAGT74800.029.4798329
TGGTATC10100.026.5454032
GAGTACG58250.026.33949512
AGTACGG58150.026.32563413
GAGTACT27700.024.0929812
AGTACTT28550.023.01419413
GTACGGG67000.022.74560514
TACGGGG28200.022.56654515
Produced by FastQC (version 0.11.5)