FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646364
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17360.2685793144420172No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8450.1307312907278252No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7990.12361455774145837No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6870.10628686003552178No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA6770.1047397441689203No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6540.1011813776757369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG252.606496E-455.026785
GTATCAA13150.043.163151
TATCAAC14300.039.4422762
CGCTAGG350.001360570439.35050623
AACGCGA350.001363162539.3352712
GGTATCA4450.038.6513751
ATCAACG15450.036.5064433
TCAACGC15400.036.401644
CAACGCA15550.036.0505035
AACGCAG16100.035.0386
AAACGCG400.002625824234.4183611
ATATCGC400.002633809334.397066
AGAGTAC16750.033.28820811
ACGCAGA17650.031.9733777
CGCAGAG18050.031.0814068
ACGGGAT1600.030.10674516
GCAGAGT18750.030.1045959
CAGAGTA18650.029.89691510
TTCGGGG2053.8060534E-828.1040170
AGCGTCG500.007810543827.5432220
Produced by FastQC (version 0.11.5)