FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231500
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGCGTCCGCGGCGGTCACGGAGGTCAGCTTCAGGGAGAACTGGTTGTTGGACGTGTCTAACGA15780.12813641900121803No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAATGCCACGAGCGTGCTCGTGCTTGTATATGAA13990.11360129922858303No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCAAATGCCACGAGCGTGCTC13510.10970361347949656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23000.040.3626371
GTGGTAT4150.038.2529451
GGTATCA9750.037.8733981
TATCAAC26950.034.5537762
TCAACGC27950.033.194114
CAACGCA28200.033.1444445
ATCAACG28500.032.6745383
AACGCAG28950.032.4049156
TGGTATC4850.032.0008352
AGAGTAC30650.030.72135211
CAGAGTA31050.030.32558610
CGCAGAG31650.029.6417168
ACGCAGA32000.029.208547
CCGTTAA500.00774631127.59183116
GCAGAGT34150.027.5727529
TAGTTAG1208.1421336E-426.81653670
CTAACGA5600.026.54433369
GAGTACG30450.025.93918612
AGTACGG34600.023.42609613
GTACGGG33550.023.3368114
Produced by FastQC (version 0.11.5)