FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231500
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGCGTCCGCGGCGGTCACGGAGGTCAGCTTCAGGGAGAACTGGTTGTTGGACGTGTCTAACGA15640.1269995939910678No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12490.10142103126268777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5050.040.9309921
GTATCAA22900.039.1138231
TATCAAC25550.034.7802772
TGGTATC5850.034.7376442
ATCAACG27100.032.7913
TCAACGC27200.032.5438164
GGTATCA10450.031.648081
CAACGCA28350.031.3451885
AACGCAG28800.031.0958716
AGAGTAC31200.028.71670711
CGCAGAG31600.028.6768638
ACGCAGA31300.028.6179877
CAGAGTA31750.028.21925410
CTAACGA5550.026.83603769
GAGTACG28950.026.3062912
GCAGAGT34350.026.283949
TACGGGG16200.025.7365915
GTACGGG31750.023.66075914
AGTACGG32550.023.60857413
TCTAACG6250.023.16786668
Produced by FastQC (version 0.11.5)