FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120410
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTCA35650.3181870922251676No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30970.2764166688979927No Hit
GTCTCCAGCCATCCTGTCTGTGTCTCCAGGAGAAGGAGTCACCCTCTCCTGCTGGGCCAGTCAGAGTCTTAGCAG21290.19001972492212674No Hit
GTATCATAGCTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAGGCGAACTGTGGCTGCACCAT19520.17422193661249008No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19510.1741326835711927No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19480.17386492444730056No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18860.16833123588686283No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18010.16074472737658538No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17840.15922742567452985No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGACT17180.15333672494890263No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGACACCAGCGGCCCTAAG17140.1529797127837131No Hit
GTGCAGCCACAGTTCGCCTGAGTTCCACCTTGGTCCCTTGGCCGAACGTC15500.13834221401094243No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15330.1368249123088869No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15140.13512910452423665No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15140.13512910452423665No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14990.13379030890477592No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14880.13280852545050473No Hit
GTCTGAAGACTTTGCACTTTATTACTGTCAGCAGTATCATAGCTGGCCTC14160.1263823064770932No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13570.12111637704054765No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12680.11317285636508063No Hit
GTCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACC12640.11281584419989112No Hit
CTATGATACTGCTGACAGTAATAAAGTGCAAAGTCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTC12400.11067377120875393No Hit
CTGCTACTCTGGCTCCCAGATGTCACTGGAGAAATTGTGATGACGCAGTC11720.10460456440053195No Hit
TTATTACTGTCAGCAGTATCATAGCTGGCCTCGGACGTTCGGCCAAGGGA11700.10442605831793718No Hit
GTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCTCTTAGGGCCGCTGGTGTCCCAGC11650.10397979311145027No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT11440.10210547924420525No Hit
GCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCTCTTAGGGCCGCTGGTGTC11430.10201622620290786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT200.00738620151.7017068
GTGGTAT2750.043.8935781
GTATCAA24150.042.1279681
GGTATCA9400.041.825641
TGGTATC3250.036.0586242
TATCAAC28950.035.122632
ATCAACG29350.034.6439553
TCAACGC29550.034.5261234
CAACGCA29950.034.180095
AACGCAG30850.033.0712136
GATTAGT800.003614037832.2459370
AGAGTAC32600.031.29591611
CGCTAAA450.004637723330.64625225
CGCAGAG33300.030.6380448
ACGCAGA33400.030.5463147
GCAGAGT34350.030.0025429
CAGAGTA35100.029.06686410
TCGCTAT1005.0468043E-727.58162524
AGTACGG28250.027.2082113
GAGCGAT1155.633774E-827.00251832
Produced by FastQC (version 0.11.5)