FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120410
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTCA32200.2873947929775707No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31130.2778447175587508No Hit
GTATCATAGCTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAGGCGAACTGTGGCTGCACCAT20120.179577119090333No Hit
GTCTCCAGCCATCCTGTCTGTGTCTCCAGGAGAAGGAGTCACCCTCTCCTGCTGGGCCAGTCAGAGTCTTAGCAG19820.17689952785141155No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19300.17225836970394767No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18680.16672468114350997No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC18480.16493962031756232No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17730.15824564222025866No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGACT17260.1540507492792817No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGACACCAGCGGCCCTAAG16330.14575021643862515No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16130.1439651556126775No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15480.13816370792834765No Hit
GTGCAGCCACAGTTCGCCTGAGTTCCACCTTGGTCCCTTGGCCGAACGTC15400.1374496835979686No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15310.13664640622629215No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT14570.1300416811702859No Hit
GTCTGAAGACTTTGCACTTTATTACTGTCAGCAGTATCATAGCTGGCCTC14440.1288813916334199No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14290.12754259601395918No Hit
CTATGATACTGCTGACAGTAATAAAGTGCAAAGTCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTC12830.11451165198454137No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12730.11361912157156755No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12220.10906721646540107No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11960.10674663739166912No Hit
CTGCTACTCTGGCTCCCAGATGTCACTGGAGAAATTGTGATGACGCAGTC11560.10317651573977384No Hit
GTCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACC11410.1018377201203131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)