FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361631
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66260.2805688102840791No Hit
GAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT43320.18343255148666324No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41560.17598007478729744No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41500.17572601308163724No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41310.1749214843470466No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40670.17221149282000447No Hit
CTCCTGTACAGCCTCTGGACTCACCTTTCGAGATTGTGCTATGAGTTGGTTACGCCAGGTTCCAGGGAAGGGA39800.1685275980879316No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38850.16450495441497848No Hit
GTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT37650.15942372030177449No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC37330.15806872453825344No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36990.15662904153951232No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA35650.15095499677976787No Hit
GTATAGTCGGTACTCGACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT34100.14439173605021277No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG33870.143417832845182No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33300.14100424664141012No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32510.13765910085021751No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAA32090.1358806689105961No Hit
CAGTATAGTCGGTACTCGACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT31660.13405989335336468No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30760.13024896776846173No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT29420.12457492300871728No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28150.11919728357224309No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27890.11809634951438222No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27350.11580979416344045No Hit
ACTATACTGTTGGCAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGC27300.11559807607539026No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT26770.11335386434205852No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTC26630.11276105369551806No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26540.11237996113702775No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24880.10535092061376226No Hit
GATCTATAAGGCGTCTAGTTTAGAAACTTGGGTCCCATCAAGGTTCAGCG24770.10488514082005192No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24770.10488514082005192No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24270.10276795993955025No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23740.10052374820621851No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23710.1003967173533884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41900.040.1945271
GTGGTAT6500.036.5601351
TATCAAC48500.034.2870672
ATCAACG48650.034.2521173
TCAACGC49550.033.6994634
CAACGCA50000.033.396175
AACGCAG50250.033.3013576
AGAGTAC55200.030.20888911
CGCAGAG56200.029.6701098
ACGCAGA56950.029.3355147
GCAGAGT58000.028.9287369
AGTACGG48550.025.68903513
GAGTACG49450.025.4305112
TGTTAGA12350.024.4058770
CGTTCGT750.001922199423.14478347
TTAACGG2700.023.02875535
TTCGGGG16000.022.44561270
TACGGGG25350.022.28919415
GTACGGG56800.021.95779414
GTTAGAC1103.3211458E-521.9093913
Produced by FastQC (version 0.11.5)