FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718797
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT60170.3500704271650462No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT58660.3412852128552703No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54060.314522308335423No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48940.2847340320002886No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC42650.2481386690807582No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42090.2448805763566029No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40040.23295362977710574No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC39240.22829921159974098No Hit
GTCTACTTCTGTGCGAGAGATCCGGCCTACAGTTATGGCTCAATGGACTT38190.22219028774194974No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG36710.21357961411382498No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34930.20322353366918838No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34450.20043088276276955No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34010.19787095276521893No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGCCCGAATGTC33030.19216929049794712No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG32900.19141294754412533No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT32650.18995844186369884No Hit
GAAGTAGACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTCCAGATACA32440.1887366570921406No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA32400.18850393618327238No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTG32390.1884457559560553No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31970.1860021864129388No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31700.1844313202780782No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT30990.180300524145667No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30420.17698425119429462No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT29440.1712825889270228No Hit
GGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG29330.17064260642763515No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29120.1694208216560769No Hit
GTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTGGATAGAGATA29050.16901356006555748No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAA28910.16819903688451865No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT28770.16738451370347981No Hit
GTCTGGGACTCCAGAGGCCCGATAGGAAAGTGTATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGAA28710.16703543234017745No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC28580.1662790893863557No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC27620.160693787573518No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC27550.16028652598299858No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG26870.15633027053223852No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26610.154817584624595No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG26320.15313035803530028No Hit
GTGTATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGAACCAGTCCACATATATGCTTCCAGTATCT25800.15010498622001317No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCA25730.14969772462949377No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25520.14847593985793553No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA25260.14696325395029197No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA24990.14539238781543137No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24830.14446150417995843No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA24740.14393788213500489No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24650.14341426009005137No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT24620.1432397194084002No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24570.14294881827231487No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC24480.14242519622736136No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG24250.14108705100136898No Hit
GGTCTAGTCAGAGCCTCGTGGATAGAGATACTGGAAGCATATATGTGGACTGGTTCCTGCAGAAGCCAGGGCAGT24170.1406216091836325No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT23880.1389343825943378No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA23360.1359090107790507No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23250.13526902827966306No Hit
GTATGGAGTTTCCCCGGACATTCGGGCAAGGGACCAGGGTGGAAATCAAA23000.13381452259923657No Hit
CTCCAGAGACAACGCCCTGAATTCACTGTATCTGGAAATGAACAGCCTGAGAGACGAGGACACGGCTGTCTACTT22920.13334908078150007No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA22550.13119641237446888No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22540.13113823214725184No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG22500.1309055112383836No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT22480.13078915078394948No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATTCATGCTATAGGTAT22440.13055642987508123No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA22280.12962554623960829No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC22120.12869466260413534No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22010.1280546801047477No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21730.12642563374267002No Hit
CTCCATACGTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACCC21690.1261929128338018No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21670.12607655237936766No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA21380.12438932579007295No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGCCCGAATGTCCGGGGAAACTC20800.1210148726114835No Hit
TCTATACACTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACA20750.1207239714753982No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGCATGCAGTGAGGATATTGTGATGA20710.12049125056652996No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCGATACCTATAGCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG20480.11915310534053761No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG20390.11862948329558407No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA20340.11833858215949877No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20070.11676771602463817No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC20070.11676771602463817No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20020.11647681488855287No Hit
GTTTATTACTGCATGCAACGTATGGAGTTTCCCCGGACATTCGGGCAAGGGACCAGGGTGGAAATCAAACGAA19800.11519684988977758No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19710.11467322784482403No Hit
ACTCCATACGTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACC19600.11403324534543637No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19510.11350962330048285No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC19470.1132769023916146No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCATCTCCTC19140.11135695489345164No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19130.11129877466623458No Hit
CCACTGTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG19060.11089151307571517No Hit
CCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT18950.1102515305763275No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC18600.10821522262373043No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC18580.10809886216929632No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC18520.10774978080599397No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCA18470.10745887966990868No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC18320.10658617626165276No Hit
GGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAACGTATGGAGTTTCCCCGGACATTCGGGCAAGGGA18310.10652799603443572No Hit
GCCTGAGAGACGAGGACACGGCTGTCTACTTCTGTGCGAGAGATCCGGCCTACAGTTATGGCTCAATGGACTT18280.10635345535278454No Hit
GCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCC18180.10577165308061395No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG18010.10478258921792394No Hit
GTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT17850.10385170558245098No Hit
GCTAATGCTCTGGGTCCCTGCATGCAGTGAGGATATTGTGATGACCCAGACTCCACTGTCCCTGCCCGTCACCC17830.10373534512801685No Hit
GCCTCATGCATCACGGAGCATGAGAAGACGTTCCCCTGCTGCCACCTGCT17760.10332808353749744No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17740.10321172308306331No Hit
GGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC17690.10292082194697802No Hit
GGTCAGGCTGACCTGGTTCTTGGTCATCTCCTCCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTG17560.10216447899315625No Hit
CTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAT17470.10164085694820271No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC17410.10129177558490038No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGCATGCAGTGAGGATATTGTGATGACCCAGACTCCACTGTCC17270.10047725240386153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA150.002296404469.4453450
TGGTATC2750.056.319732
GTGGTAT2900.054.6014021
GGTATCA9900.046.5922361
GTCTATA350.001362965139.3401371
GTATCAA23600.039.2359541
TATCAAC25850.035.815622
GTAAATT601.1096568E-535.47436566
TACACCG601.3651021E-534.4176145
ATCAACG28900.032.1548653
TCAACGC28800.032.1470074
GGCTTAC751.6994018E-632.127781
CAACGCA29300.031.5984255
AACGCAG29750.031.2388826
TAAATTC702.9613107E-530.73280767
TGTATAA802.8146624E-630.1154142
AGAGTAC31250.029.64565711
CGCAGAG31450.029.5649158
CAGAGTA31600.029.42628710
GCAGAGT32700.028.4364139
Produced by FastQC (version 0.11.5)