FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855276
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAACCTTCACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGA30770.3597669056538474No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23610.27605123959984845No Hit
GGTTTATACTTTGCAGGCAGTAATAAACCCCAACATCCTCGGCCTCCACCCGGCTGATTTTCAGTGTGAAAT21780.25465463780113085No Hit
GTGATGGAAAGACCTATTTGTATTGGTACTTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGGAGTTT18440.2156029164854386No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17410.20356002039107843No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17240.2015723579289025No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16140.18871101258541104No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15220.1779542510254No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15180.17748656574018212No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14480.16930207324886937No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14240.16649596153756216No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC13530.15819454772494493No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATGTTGTGATGA12600.1473208648436294No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12580.1470870222010205No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGTAAGTCTAGTCGGAGCCTCTTG12540.14661933691580262No Hit
GTGCAGATGTTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCT12160.14217632670623284No Hit
TTCCATCACTATGCAAGAGGCTCCGACTAGACTTACAGGAGATGGAGGCC11870.1387856083884033No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG11860.1386686870670988No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA11620.13586257535579158No Hit
GGATACCTGGATCCAGTGCAGATGTTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGG11450.1338749128936156No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11230.13130264382491735No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11070.12943190268404586No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATGTTGTGATGACCCAGACTCCACT11060.1293149813627414No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10970.1282626894710012No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10920.12767808286447885No Hit
CCTGGATCCAGTGCAGATGTTGTGATGACCCAGACTCCACTCTCTCTGTC10870.12709347625795647No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCCCCTGGCCAAAAGTG10790.12615810568752076No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATGTTGTGATGACCCAGACTCCACTCTCT10520.12300123001230012No Hit
CTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCA10420.12183201679925545No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10350.12101356755012417No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10300.12042896094360182No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCC10070.11773977055359906No Hit
GTAATAAACCCCAACATCCTCGGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA10040.11738900658968567No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9810.11469981619968292No Hit
GTGGAGGCTGGCCTGGCTTCTGCAAGTACCAATACAAATAGGTCTTTCCA9690.11329676034402929No Hit
CTCTTGCATAGTGATGGAAAGACCTATTTGTATTGGTACTTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGAT9450.11049064863272205No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9430.11025680599011313No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9300.10873682881315505No Hit
GTATTGGTACTTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATG9290.10861990749185058No Hit
GCTAATGCTCTGGATACCTGGATCCAGTGCAGATGTTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCC9280.10850298617054613No Hit
AGCCTCCACAGCTCCTGATCTATGGAGTTTCCTACCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGT9160.1070999303148925No Hit
GGCCAAAAGTGTGAAGGTTTATACTTTGCAGGCAGTAATAAACCCCAACA9120.10663224502967462No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATT9070.1060476384231523No Hit
GGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCCGAGGAT8710.10183847085619144No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT8700.10172154953488699No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8590.10043541500053783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)