FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855276
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTATACTTTGCAGGCAGTAATAAACCCCAACATCCTCGGCCTCCACCCGGCTGATTTTCAGTGTGAAAT29490.3448009765268755No Hit
GTATAAACCTTCACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGA28200.3297181260785992No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24570.2872756864450774No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16300.1905817537262825No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16210.1895294618345423No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15620.1826311038775787No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15500.1812280480219251No Hit
GTAATAAACCCCAACATCCTCGGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA15430.18040959877279383No Hit
GTGATGGAAAGACCTATTTGTATTGGTACTTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGGAGTTT14220.1662621188949532No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14030.16404061379016832No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13760.1608837381149477No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC13030.15234848165972154No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12020.14053942820797027No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11880.13890252970970776No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCCCCTGGCCAAAAGTG11750.13738255253274964No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11530.13481028346405138No Hit
TTCCATCACTATGCAAGAGGCTCCGACTAGACTTACAGGAGATGGAGGCC11410.13340722760839777No Hit
GGCCAAAAGTGTGAAGGTTTATACTTTGCAGGCAGTAATAAACCCCAACA11340.1325887783592665No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11000.1286134534349146No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA10960.12814576814969672No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCC10690.12498889247447607No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10360.12113048887142865No Hit
CTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCA10330.12077972490751522No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATGTTGTGATGA10290.12031203962229738No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10220.11949359037316609No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG10130.11844129848142587No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10050.11750592791099014No Hit
GTGCAGATGTTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCT9780.11434905223576951No Hit
GTGGAGGCTGGCCTGGCTTCTGCAAGTACCAATACAAATAGGTCTTTCCA9490.11095833391793994No Hit
GTCCCTGACCCGCTGCCACTGAACCTATCTGGCACTCCAGAGAACCGGTA9390.10978912070489526No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9360.10943835674098186No Hit
GGATACCTGGATCCAGTGCAGATGTTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGG9220.1078014582427193No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATGTTGTGATGACCCAGACTCCACT9070.1060476384231523No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9050.10581379578054335No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8980.10499534653141207No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8970.10487842521010762No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8760.1024230774627138No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATGTTGTGATGACCCAGACTCCACTCTCT8740.10218923482010485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8710.10183847085619144No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8710.10183847085619144No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGTAAGTCTAGTCGGAGCCTCTTGCATAGTGATGGAAAGACCTATTTGT8680.10148770689227804No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8660.10125386424966912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)