FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195819
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24470.20462963040393237No Hit
GTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21760.18196733786634933No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20260.16942363350975356No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19710.16482427524566845No Hit
GAGTTACGGTAACCCGTACAGTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19320.16156291211295357No Hit
GGCCAAAACTGTACGGGTTACCGTAACTCTGTTGACAGTAGTAAGTTGCA18570.15529105993465567No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18120.15152794862767693No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16250.13589013052978755No Hit
GTCCCAGACCCACCGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT15720.1314580216571237No Hit
GTCTGGGACAGATTTCGCTCTCACCATCAGCAGTCTGCAACCTGACGATT15670.1310398981785705No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15530.12986915243862157No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14870.12434992252171942No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14770.12351367556461305No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCGGTGGGTCTGGGACAGATTTCG14590.12200843104182155No Hit
GCATAGATCAGGAGCTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAATT14550.12167393225897899No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14130.11816169503913218No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13010.10879572911954066No Hit
CCGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGGTTGCAGACTGCTGATGGTGAGAGCGAAATCTGTC12790.10695598581390661No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGGGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGCAG12490.10444724494258746No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCAGGCCAAAACTG12430.10394549676832363No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12220.10218937815840022No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11990.10026601015705554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19750.037.2200281
TATCAAC23200.031.6586592
ATCAACG23550.031.1881473
CAACGCA23850.031.0850055
TCAACGC23750.030.9255084
TGTTAGA5900.030.1291170
GTGGTAT3800.029.9705371
AACGCAG24900.029.9126766
ACGCAGA26900.027.9450637
AGAGTAC26400.027.56462111
CGCAGAG28850.025.8204218
CCCTATA700.001337789524.6304782
GCAGAGT30500.024.5366489
AGTACGG28450.024.3661713
CAGAGTA30800.023.96274410
GAGTACG29850.023.45444712
TATACAC1403.794321E-722.1674293
TCGCTAT1103.2723445E-521.9499424
GTGTTAG5950.021.59358269
TTTCGCA850.003510340120.86316566
Produced by FastQC (version 0.11.5)