FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052883
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAGAGTTATCCGTGGACGTTCGGCCAAGGGACCAAGTTGGAAATCAAACGAACTGTGGCTGCACCAT27840.2644168440368018No Hit
CTCTCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC25110.23848803713233097No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20680.19641308673423352No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAATCCAGGGTACGCCCCTAAGCTTCTGATCTATAAGGCGT16380.1555728414268252No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16010.1520586807840947No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15350.14579017801598088No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13360.12688969239697098No Hit
CCCCTAAGCTTCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCA12550.11919652990883128No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12430.11805680213281058No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12390.11767689287413702No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12340.11720200630079505No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12200.11587232389543758No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11890.11292802714071744No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAACTTGGTCCCTTGGCCGAACGTC11540.10960382112732373No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11260.10694445631660876No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGAGAGTTAT11070.10513988733790935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29850.037.8470421
GTGGTAT4700.037.2608451
CCGCTAT901.7080337E-435.00481870
TATCAAC33350.033.646782
CGAGACG1850.033.4040123
TCAACGC35700.031.3358084
ATCAACG35750.031.1959973
CAACGCA37100.030.3383165
AACGCAG38150.029.5932676
TCGTATG605.383433E-428.78497740
ACGAGAC2300.028.35709422
AGAGTAC40650.027.94607711
TCGACGG500.00755878527.73034944
ACGCAGA41150.027.519197
AGTACGG31450.027.28178413
CGCAGAG41750.027.2084858
GAGTACG32250.026.71144512
TACGGGG16850.026.47619815
GCAGAGT43700.026.1514389
TATACAC2950.025.591293
Produced by FastQC (version 0.11.5)