FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052883
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAGAGTTATCCGTGGACGTTCGGCCAAGGGACCAAGTTGGAAATCAAACGAACTGTGGCTGCACCAT26400.25074011072455343No Hit
CTCTCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC24620.23383414871357977No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21970.20866516032645605No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15710.14920936134404297No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAATCCAGGGTACGCCCCTAAGCTTCTGATCTATAAGGCGT15080.1432257905199343No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14080.13372805905309518No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13870.13173353544505895No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13860.13163855813039055No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13790.13097371692771181No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12610.11976639379684163No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12600.11967141648217325No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAACTTGGTCCCTTGGCCGAACGTC12070.11463761880474849No Hit
CCCCTAAGCTTCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCA11320.10751432020461912No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11220.10656454705793522No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10770.1022905678978576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33500.039.6874471
TATCAAC36250.036.5699842
GTGGTAT5300.034.9127541
TCAACGC37650.034.846224
ATCAACG37750.034.8446463
CAACGCA38550.034.1215485
AACGCAG39400.033.5624926
ACGCAGA42200.031.2648077
AGAGTAC42400.031.2040911
CGCAGAG42800.030.7522938
GCAGAGT45650.029.0576329
AGTACGG35650.027.59385713
GAGTACG35950.027.07755912
TACGGGG19100.026.20049715
GTACGGG38800.024.91193214
CAGAGTA53950.024.6507610
GGTATCA23850.024.4245621
AGTACTT14550.023.79295713
CCGCATA750.002026186622.9345834
ACGGGGC4500.022.84740616
Produced by FastQC (version 0.11.5)