FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146738
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26750.039.374571
GTGGTAT5000.034.420581
GTACCGT400.002626963234.417586
TATCAAC30700.033.6284142
ATCAACG30700.033.4042243
GGTATCA11850.033.403941
TCAACGC30800.032.9605754
CAACGCA31550.032.1770485
AACGCAG32700.031.2600046
CTCGACG450.004565413630.7449139
ACGCAGA34650.029.3149097
AGAGTAC35400.028.70134211
CGCAGAG35800.028.1845558
CAGAGTA37000.027.27284210
TGGTATC6150.026.8589862
GCAGAGT38350.026.4025869
TTAACGG3100.024.50767135
AGTACGG29600.024.08585513
GAGTACG29950.023.80438612
TAACGGC3200.023.75012236
Produced by FastQC (version 0.11.5)