FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817744
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA51830.2851336601853727No Hit
CCCAAGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCC45120.2482197713209341No Hit
CCCCTGCACTGGGAGCAGTTCCAACATCGGGGCAGATTATGATGTACACTGGTACCAGCAACTTCCAGGAGCAGC41750.2296803070179299No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGAACCACTC37420.2058595709846931No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG37070.20393410733304582No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33920.18660493446821996No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31330.1723565034460298No Hit
GTTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC28730.15805305917664975No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC28130.15475226434525433No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27250.1499110985925411No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26780.14732547597461468No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26280.1445748136151185No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26050.14330950892975028No Hit
GTTCCAACATCGGGGCAGATTATGATGTACACTGGTACCAGCAACTTCCA26010.1430894559409906No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC24900.1369829855029091No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24610.13538760133440134No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24280.13357216417713386No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCTTGGGTGTTCGGCGGAGGGACCA24240.13335211118837417No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23450.12900606466037023No Hit
GATTATGATGTACACTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGG23050.1268055347727733No Hit
GGGCAGAGGGTCACCATCCCCTGCACTGGGAGCAGTTCCAACATCGGGGCAGATTATGATGTACACTGGTACCAG22730.12504511086269574No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22690.12482505787393605No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTT22530.12394484591889726No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21810.11998389212122279No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT21720.11948877289651347No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21660.11915869341337394No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAACTCCTCAT21430.1178933887280057No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCCCCTGCACTGGGAGCAG21230.11679312378420723No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21030.11569285884040877No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20730.11404246142471108No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20330.11184193153711414No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20130.11074166659331566No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC19050.10480023589680394No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC18730.1030398119867264No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGAACCACTCA18710.10292978549234655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.043.0411381
GTATCAA48100.041.061381
GTGGTAT6750.040.851711
TGGTATC7300.036.329322
TATCAAC54650.036.2382352
TCAACGC55450.035.5290684
ATCAACG55600.035.433223
CAACGCA57250.034.472165
AACGCAG57900.034.204146
TCGTATG1305.456968E-1231.92265740
ACGCAGA63000.031.4881697
CGCAGAG63100.031.4382678
AGAGTAC63500.031.4029411
CAGAGTA65350.030.61935610
GCAGAGT65550.030.3683139
AGTACGG47650.028.76640913
GAGTACG48150.028.5392212
CGTATGC1503.274181E-1127.68388741
TATAGCG500.00779977127.5536635
TACGGGT4600.026.95318815
Produced by FastQC (version 0.11.5)