FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817744
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA41840.23017542624263923No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCCCCTG38970.21438662429913125No Hit
CCCCTGCACTGGGAGCAGTTCCAACATCGGGGCAGATTATGATGTACACTGGTACCAGCAACTTCCAGGAGCAGC37450.20602461072626288No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC32070.17642748373808412No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31780.17483209956957635No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC31450.1730166624123089No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG30970.17037602654719256No Hit
GTTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC30090.16553486079447932No Hit
CCCAAGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCC28660.15766796644632028No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGAACCACTC26350.144959906345448No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25710.1414390585252929No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25420.1398436743567851No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24760.13621280004225017No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24640.1355526410759711No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCTTGGGTGTTCGGCGGAGGGACCA24550.1350575218512618No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23900.13148166078391677No Hit
GTTCCAACATCGGGGCAGATTATGATGTACACTGGTACCAGCAACTTCCA23290.12812585270533144No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC23180.1275207069862423No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22970.1263654287952539No Hit
GGGCAGAGGGTCACCATCCCCTGCACTGGGAGCAGTTCCAACATCGGGGCAGATTATGATGTACACTGGTACCAG22880.1258703095705446No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC22720.12499009761550582No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22530.12394484591889726No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT21810.11998389212122279No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTT21710.11943375964932357No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21640.1190486669189941No Hit
GATTATGATGTACACTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGG21500.11827848145833517No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC21080.11596792507635838No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20470.11261211699777306No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20150.1108516930876955No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAACTCCTCAT19720.10848612345852882No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19530.10744087176192028No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC19390.10667068630126134No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCCCCTGCACTGGGAGCAG19300.10617556707655203No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC19150.10535036836870318No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19070.10491026239118378No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18890.10392002394176518No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18620.10243466626763725No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18260.1004541893688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)