FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774589
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44800.042.773121
GGTATCA16600.041.3160861
TATCAAC49350.038.666932
ATCAACG52450.036.2502253
TCAACGC52500.036.1500934
CAACGCA53450.035.70095
AACGCAG54200.035.2068866
ACGCAGA59000.032.4009747
AGAGTAC60300.031.64800311
TTATACA6400.031.2151282
CGCAGAG61200.031.1245528
CTTATAC6550.031.0445161
CAGTTCG6200.030.97419470
CAGAGTA62550.030.50958310
TATACAC6600.029.7473343
GCAGAGT65600.029.1419419
AGTACGG54950.027.70820413
GTGGTAT8100.027.6568371
GAGTACG56600.027.0221812
TCGTATG5050.026.72629540
Produced by FastQC (version 0.11.5)