FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717288
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTTCCTCCGCCGAATACGAC23450.3269258652033772No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18230.25415174936706036No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCTCCTG13950.19448255094188108No Hit
GTCTCTGGTGGCTCCGTCGGCAGTGACAGTGACACTAACTACTGGAACTGGATCCGGCAGCCCCCAGGGAAGGGA13670.19057895852154225No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTTCCTC12410.1730127926300175No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATAACTATGTCTCCTGGTATCAACTGCACCCAGGCAAAGC12020.16757564604454556No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11840.16506619377432774No Hit
CTCCTGGACAGTCGATCGCCATCTCCTGCACTGGAACCAGCAGTGACATT11790.16436912369926723No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10900.15196127636319023No Hit
GACCCAGGACGGTCAGCTTGGTTCCTCCGCCGAATACGACATCCAAAGTG10710.1493124100779603No Hit
TCTCTACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCC10010.13955342902711323No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9520.1327221422915203No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9350.13035210403631456No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9090.12672733964599994No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9040.12603026957093943No Hit
GGTATCAACTGCACCCAGGCAAAGCCCCCAAACTCATAATTTTTGAGGTC9020.12575144154091522No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGC8890.1239390593457579No Hit
GTGATAGTCAGCCTCGTCTTCAGCCTGGAGCCCGGAGATGGTCAGGGAGG8880.1237996453307458No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8790.1225449191956369No Hit
GTCTATTACTGTGCGAGAACTTCCACTCTAGTAGGATTACCCTTTGACTT8790.1225449191956369No Hit
GACTATCACTGCAGCTCATATACAAGCAGTAGCACTTTGGATGTCGTATTCGGCGGAGGAACCAAGCTGACCGTC8770.12226609116561268No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8650.1205931229854675No Hit
GTGCTACTGCTTGTATATGAGCTGCAGTGATAGTCAGCCTCGTCTTCAGC8310.11585304647505604No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG8230.11473773435495924No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCT8200.11431949230992294No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8010.11167062602469302No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7870.10971882981452359No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC7790.10860351769442678No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7630.10637289345423316No Hit
ACCCAGGACGGTCAGCTTGGTTCCTCCGCCGAATACGACATCCAAAGTGC7560.10539699534914847No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7470.10414226921403955No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7470.10414226921403955No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7320.10205105898885804No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7290.10163281694382173No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7280.10149340292880964No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7260.10121457489878542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC200.007329087251.80143
CGTTCGT200.00733110851.79778723
ATCAACG15200.035.2158623
TCAACGC15200.035.2158624
CAACGCA15600.034.3128935
AACGCAG15600.034.3128936
AGAGTAC16650.032.3541711
CTACGCT751.6533959E-632.23647728
CGCAGAG16900.031.875568
ACGCAGA16800.031.859757
CAGAGTA17100.031.50274710
TACGGGT1359.094947E-1230.69498415
GCAGAGT17750.030.3491259
ACTACGC802.7369788E-630.2195927
TATACAC1202.6539055E-928.7785553
CGGGATA1700.028.43800217
GTATATG12450.028.29865670
GTGGTAT1503.45608E-1127.6428281
AGTACGG15350.026.99558813
GAGTACG15400.026.90794212
Produced by FastQC (version 0.11.5)