FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529623
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6080.11479863978716937No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC5530.10441389441168529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16950.041.4088631
GGTATCA6750.040.2676351
GTATTAT350.001365370239.3210221
TCAACGC19700.035.269154
CAACGCA20000.034.912095
ATCAACG20050.034.6534773
AACGCAG20300.034.5721176
TATCAAC21150.033.0137982
ACGCAGA21400.032.640457
CGCAGAG21500.032.1807068
GTGGTAT2550.031.0327661
CAGAGTA22450.030.81893710
AGAGTAC22250.030.78654911
GCAGAGT24000.029.1153989
TATAGTG500.00784578527.5169185
AGTACGG22550.027.47665813
GAGTACG22650.027.35534912
TGGTATC3050.027.065822
TACGGGT3600.025.80929615
GTCGTGC851.464136E-424.2979858
Produced by FastQC (version 0.11.5)